PDF(Pubmed)
Abstract：
UNASSIGNED: Estrogen deficiency is associated with unfavorable changes in body composition and metabolic health. While physical activity ameliorates several of the negative effects, loss of ovarian function is associated with decreased physical activity levels. It has been proposed that the changes in brain neurochemical levels and /or impaired skeletal muscle function may underlie this phenomenon.
UNASSIGNED: We studied the effect of estrogen deficiency induced via ovariectomy (OVX) in female Wistar rats (n = 64). Rats underwent either sham or OVX surgery and were allocated thereafter into four groups matched for body mass and maximal running capacity: sham/control, sham/max, OVX/control, and OVX/max, of which the max groups had maximal running test before euthanasia to induce acute response to exercise. Metabolism, spontaneous activity, and maximal running capacity were measured before (PRE) and after (POST) the surgeries. Three months following the surgery, rats were euthanized, and blood and tissue samples harvested. Proteins were analyzed from gastrocnemius muscle and retroperitoneal adipose tissue via Western blot. Brain neurochemical markers were measured from nucleus accumbens (NA) and hippocampus (HC) using ultra-high performance liquid chromatography.
UNASSIGNED: OVX had lower basal energy expenditure and higher body mass and retroperitoneal adipose tissue mass compared with sham group (p ≤ 0.005). OVX reduced maximal running capacity by 17% (p = 0.005) with no changes in muscle mass or phosphorylated form of regulatory light chain (pRLC) in gastrocnemius muscle. OVX was associated with lower serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) level in the NA compared with sham (p = 0.007). In response to acute exercise, OVX was associated with low serotonin level in the HC and high level in the NA (p ≤ 0.024).
UNASSIGNED: Our results highlight that OVX reduces maximal running capacity and affects the response of brain neurochemical levels to acute exercise in a brain region-specific manner. These results may offer mechanistic insight into why OVX reduces willingness to exercise.
UNASSIGNED: We studied the effect of estrogen deficiency induced via ovariectomy (OVX) in female Wistar rats (n = 64). Rats underwent either sham or OVX surgery and were allocated thereafter into four groups matched for body mass and maximal running capacity: sham/control, sham/max, OVX/control, and OVX/max, of which the max groups had maximal running test before euthanasia to induce acute response to exercise. Metabolism, spontaneous activity, and maximal running capacity were measured before (PRE) and after (POST) the surgeries. Three months following the surgery, rats were euthanized, and blood and tissue samples harvested. Proteins were analyzed from gastrocnemius muscle and retroperitoneal adipose tissue via Western blot. Brain neurochemical markers were measured from nucleus accumbens (NA) and hippocampus (HC) using ultra-high performance liquid chromatography.
UNASSIGNED: OVX had lower basal energy expenditure and higher body mass and retroperitoneal adipose tissue mass compared with sham group (p ≤ 0.005). OVX reduced maximal running capacity by 17% (p = 0.005) with no changes in muscle mass or phosphorylated form of regulatory light chain (pRLC) in gastrocnemius muscle. OVX was associated with lower serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) level in the NA compared with sham (p = 0.007). In response to acute exercise, OVX was associated with low serotonin level in the HC and high level in the NA (p ≤ 0.024).
UNASSIGNED: Our results highlight that OVX reduces maximal running capacity and affects the response of brain neurochemical levels to acute exercise in a brain region-specific manner. These results may offer mechanistic insight into why OVX reduces willingness to exercise.
摘要：
■雌激素缺乏与身体成分和代谢健康的不利变化有关。虽然体力活动改善了一些负面影响，卵巢功能丧失与体力活动水平下降有关。已经提出，脑神经化学水平的变化和/或骨骼肌功能受损可能是这种现象的基础。
■我们研究了雌性Wistar大鼠（n=64）卵巢切除术（OVX）引起的雌激素缺乏的影响。大鼠接受假手术或OVX手术，然后分为四组，其体重和最大跑步能力相匹配：假/对照组，sham/max,OVX/控件,和OVX/max，其中max组在安乐死前进行了最大跑步测试，以诱导对运动的急性反应。代谢，自发活动，并在手术前（PRE）和手术后（POST）测量最大运行能力。手术后三个月,对大鼠实施安乐死,采集血液和组织样本.通过Western印迹分析来自腓肠肌和腹膜后脂肪组织的蛋白质。使用超高效液相色谱法测量伏隔核（NA）和海马（HC）的脑神经化学标记。
■与假手术组相比，OVX的基础能量消耗较低，体重和腹膜后脂肪组织质量较高（p≤0.005）。OVX使最大运行能力降低了17％（p=0.005），而腓肠肌中的肌肉质量或调节轻链（pRLC）的磷酸化形式没有变化。与假手术相比，OVX与NA中5-羟色胺代谢物5-羟基吲哚乙酸（5-HIAA）水平较低相关（p=0.007）。为了应对急性运动，OVX与HC中5-羟色胺水平低和NA中的高水平相关（p≤0.024）。
■我们的研究结果突出表明，OVX会降低最大跑步能力，并以特定于大脑区域的方式影响大脑神经化学水平对急性运动的反应。这些结果可能为OVX降低运动意愿的原因提供了机械见解。
■我们研究了雌性Wistar大鼠（n=64）卵巢切除术（OVX）引起的雌激素缺乏的影响。大鼠接受假手术或OVX手术，然后分为四组，其体重和最大跑步能力相匹配：假/对照组，sham/max,OVX/控件,和OVX/max，其中max组在安乐死前进行了最大跑步测试，以诱导对运动的急性反应。代谢，自发活动，并在手术前（PRE）和手术后（POST）测量最大运行能力。手术后三个月,对大鼠实施安乐死,采集血液和组织样本.通过Western印迹分析来自腓肠肌和腹膜后脂肪组织的蛋白质。使用超高效液相色谱法测量伏隔核（NA）和海马（HC）的脑神经化学标记。
■与假手术组相比，OVX的基础能量消耗较低，体重和腹膜后脂肪组织质量较高（p≤0.005）。OVX使最大运行能力降低了17％（p=0.005），而腓肠肌中的肌肉质量或调节轻链（pRLC）的磷酸化形式没有变化。与假手术相比，OVX与NA中5-羟色胺代谢物5-羟基吲哚乙酸（5-HIAA）水平较低相关（p=0.007）。为了应对急性运动，OVX与HC中5-羟色胺水平低和NA中的高水平相关（p≤0.024）。
■我们的研究结果突出表明，OVX会降低最大跑步能力，并以特定于大脑区域的方式影响大脑神经化学水平对急性运动的反应。这些结果可能为OVX降低运动意愿的原因提供了机械见解。
