PDF(Pubmed)
Abstract：
Early diagnosis of infectious uveitis can lead to prompt initiation of treatment to minimize vision-threatening sequelae. As various infectious etiologies of uveitis share similar clinical features, advancements in polymerase chain reaction (PCR) and metagenomic next-generation sequencing (MDS) have shown significant promise in improving diagnostic capabilities. Various techniques of PCR, including real-time, multiplex, comprehensive, and broad-range, have increased the armamentarium for infectious uveitis diagnosis. Additionally, metagenomic deep sequencing technology has provided a methodology to identify causative pathogens as well as novel etiologies of uveitis. This review discusses the diagnostic tools available for infectious uveitis and highlights the advantages and disadvantages of the techniques.
摘要：
感染性葡萄膜炎的早期诊断可以导致迅速开始治疗，以最大程度地减少威胁视力的后遗症。由于葡萄膜炎的各种感染性病因具有相似的临床特征，聚合酶链反应（PCR）和宏基因组下一代测序（MDS）的进步在提高诊断能力方面显示出重大希望。各种PCR技术，包括实时，多路复用，全面,和广泛的范围，增加了用于感染性葡萄膜炎诊断的医疗设备。此外,宏基因组深度测序技术提供了一种方法来鉴定致病病原体以及葡萄膜炎的新病因。这篇综述讨论了感染性葡萄膜炎的诊断工具，并强调了该技术的优缺点。
