PDF(Pubmed)
Abstract：
Omega-3 polyunsaturated fatty acids (n-3 PUFAs), mainly including α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), possess antioxidant properties and play a crucial role in growth and development. However, the combined effects of ALA, EPA, and DHA at different concentrations have rarely been reported. This work explored the effects of EPA, ALA, and DHA on the viability and antioxidant capacity of mouse hepatocytes, with the objective of enhancing the antioxidant capacity. Within the appropriate concentration range, cell viability and the activity of glutathione S-transferase, superoxide dismutase, and catalase were increased, while the oxidation products of malondialdehyde and the level of intracellular reactive oxygen species were obviously reduced. Thus, oxidative stress was relieved, and cellular antioxidant levels were improved. Finally, response surface optimization was carried out for EPA, ALA, and DHA, and the model was established. The antioxidant capacity of the cells was highest at EPA, ALA, and DHA concentrations of 145.46, 405.05, and 551.52 µM, respectively. These findings lay the foundation for further exploration of the interactive mechanisms of n-3 PUFAs in the body, as well as their applications in nutraceutical food.
摘要：
ω-3多不饱和脂肪酸（n-3PUFA），主要包括α-亚麻酸（ALA），二十碳五烯酸（EPA），二十二碳六烯酸（DHA），具有抗氧化性能，在生长发育中起着至关重要的作用。然而,ALA的综合作用，EPA,不同浓度的DHA很少有报道。这项工作探索了EPA的影响，ALA,和DHA对小鼠肝细胞的活力和抗氧化能力，目的是增强抗氧化能力。在适当的浓度范围内，细胞活力和谷胱甘肽S-转移酶的活性，超氧化物歧化酶,过氧化氢酶增加了，丙二醛的氧化产物和细胞内活性氧水平明显降低。因此，氧化应激得到缓解，细胞抗氧化剂水平得到改善。最后,对EPA进行了响应面优化，ALA,还有DHA,并建立了模型。细胞的抗氧化能力在EPA最高，ALA,和DHA浓度为145.46、405.05和551.52µM，分别。这些发现为进一步探索n-3PUFA在体内的相互作用机制奠定了基础，以及它们在营养食品中的应用。
