PDF(Pubmed)
Abstract：
Red blood cells (RBCs) express the nucleic acid-binding toll-like receptor 9 (TLR9) and bind CpG-containing DNA. However, whether human RBCs express other nucleic acid-binding TLRs is unknown. Here we show that human RBCs express the RNA sensor TLR7. TLR7 is present on the red cell membrane and is associated with the RBC membrane protein Band 3. In patients with SARS-CoV2-associated sepsis, TLR7-Band 3 interactions in the RBC membrane are increased when compared with healthy controls. In vitro, RBCs bind synthetic ssRNA and RNA from ssRNA viruses. Thus, RBCs may serve as a previously unrecognized sink for exogenous RNA, expanding the repertoire of non-gas exchanging functions performed by RBCs.
摘要：
红细胞(RBC)表达核酸结合toll样受体9(TLR9)并结合含CpG的DNA。然而,人红细胞是否表达其他结合核酸的TLR是未知的。在这里，我们显示人RBC表达RNA传感器TLR7。TLR7存在于红细胞膜上并且与RBC膜蛋白带3相关。在SARS-CoV2相关脓毒症患者中，与健康对照相比,RBC膜中的TLR7-带3相互作用增加。体外，RBC结合合成的ssRNA和来自ssRNA病毒的RNA。因此，红细胞可以作为一个以前未被识别的外源RNA的汇，扩大红细胞执行的非气体交换功能。
