Abstract：
Polysialylated neural cell adhesion molecule (PSA-NCAM) is expressed in the developing central nervous system (CNS) and plays an important role in neurogenesis. Organophosphorus (OP) toxins, including diazinon (DZN), cause oxidative stress (OS) and damage the CNS. Resveratrol (RV), with its antioxidant effect, leads to the reduction of OS. Therefore, this research was conducted with the aim of the effect of RVon the expression of PSA-NCAM in the hippocampus (HPC) of rat fetuses treated with DZN. In this study, 24 female Wistar rats were divided into 4 groups (n = 6): Control, DZN (40 mg/kg), RV(10 mg/kg), and DZN + RV(40 mg/kg + 10 mg/kg) after confirming they were pregnant. On the 21st day of pregnancy, the mother mice were anesthetized with ketamine and xylazine, and the fetuses were removed; after anesthesia, their brains were removed for immunohistochemistry and western blot (WB) technique. The results of the study showed that in the group receiving DZN, the level of PSA-NCAM protein expression decreased significantly compared to the control group, and the group receiving RV with its antioxidant property increased the expression of PSA-NCAM protein compared to the DZN group. All in all, the exposure of pregnant mice to DZN causes disorders in the CNS, especially the level of PSA-NCAM protein expression in the HPC of fetuses, and the use of RV as an antioxidant by pregnant mothers neutralizes the effects of DZN in the HPC of their fetuses.
摘要：
多唾液酸化神经细胞粘附分子（PSA-NCAM）在发育中的中枢神经系统（CNS）中表达，在神经发生中起重要作用。有机磷（OP）毒素，包括二嗪农（DZN），引起氧化应激（OS）并损伤中枢神经系统。白藜芦醇（RSV），以其抗氧化作用，导致操作系统的减少。因此,这项研究的目的是研究RSV对用DZN治疗的大鼠胎儿海马（HPC）中PSA-NCAM表达的影响。在这项研究中,将24只雌性Wistar大鼠分为4组（n=6）：对照组，DZN（40mg/kg），RSV（10mg/kg），和DZN+RSV(40mg/kg+10mg/kg)在确认他们怀孕后。在怀孕的第21天，用氯胺酮和赛拉嗪麻醉母鼠，胎儿被切除；麻醉后，他们的大脑被切除用于免疫组织化学和蛋白质印迹（WB）技术。研究结果表明，在接受DZN的组中，与对照组相比，PSA-NCAM蛋白表达水平明显下降，与DZN组相比，接受具有抗氧化特性的RSV组增加了PSA-NCAM蛋白的表达。总而言之，怀孕小鼠暴露于DZN会导致中枢神经系统紊乱，尤其是胎儿HPC中PSA-NCAM蛋白的表达水平，孕妇使用RSV作为抗氧化剂可以中和DZN在胎儿HPC中的作用。
