Abstract：
Intravenous regional limb perfusion (IVRLP) has been used in the treatment of pododermatitis and distal limb infections, which are significant causes of morbidity in avian species. This intravenous drug administration technique is designed to achieve high drug tissue concentrations while minimizing systemic toxic effects. Amikacin is commonly used for IVRLP in veterinary medicine, but dosing guidelines have not been established for its use in birds. The current study aimed to determine the tissue concentration of amikacin after a single IVRLP administration in healthy, euhydrated leghorn hen chickens (Gallus gallus domesticus). Chickens received a single IVRLP dose of 10 mg/kg amikacin and were euthanatized posttreatment at 1 hour (n = 6), 12 hours (n = 6), and 24 hours (n = 6) to assess tissue and synovial fluid concentrations of amikacin in the injected leg. Mean tissue concentrations were highest 1 hour post-IVRLP (synovial fluid = 153.0 µg/mL, metatarsal pad tissue = 26.05 µg/mL) before declining at the 12- and 24-hour time points. This indicates that administration of amikacin via IVRLP can reach minimum inhibitory concentrations of common bacterial isolates in tissues after a single treatment with 10 mg/kg amikacin. Regional limb perfusion every 24 hours is recommended, although the minimum days of treatment may be case dependent and vary based on response to therapy.
摘要：
静脉局部肢体灌注（IVRLP）已用于治疗足皮炎和远端肢体感染，这是鸟类发病的重要原因。这种静脉内给药技术被设计成实现高药物组织浓度,同时使全身毒性作用最小化。阿米卡星通常用于兽医学中的IVRLP，但是还没有建立用于鸟类的剂量指南。本研究旨在确定健康人群中单次IVRLP给药后阿米卡星的组织浓度,全水莱格霍恩母鸡（家鸡）。鸡接受单次IVRLP剂量为10mg/kg阿米卡星，并在1小时后进行安乐死（n=6），12小时（n=6），和24小时（n=6）以评估注射腿中阿米卡星的组织和滑液浓度。IVRLP后1小时平均组织浓度最高（滑液=153.0µg/mL，跖骨垫组织=26.05µg/mL），然后在12小时和24小时时间点下降。这表明通过IVRLP施用阿米卡星可以在用10mg/kg阿米卡星单次处理后达到组织中常见细菌分离株的最小抑制浓度。建议每24小时局部肢体灌注一次，尽管最小治疗天数可能取决于病例，并且根据对治疗的反应而有所不同。
