Abstract：
Rhipicephalus microplus poses a significant economic threat due to its role in transmitting Babesia bigemina, B. bovis and Anaplasma marginale. Chemical control methods, commonly employed, encounter challenges like resistance, high costs, and environmental concerns. Emerging as an alternative, entomopathogenic fungi, particularly Beauveria bassiana, present a promising avenue for biological control. Molecular identification using the internal transcribed spacer (ITS1-5.8-ITS4) region ensures accurate species identification. This study investigated two B. bassiana strains, assessing their molecular characterization, impact on R. microplus mortality, and reproductive effects on adult females. The Reproductive Aptitude Index (RAI) is employed to evaluate tick egg viability post-treatment, providing insights into the potential of these fungi for tick control. Results indicate the BbLn2021-1 strain causes 96% mortality, and BbSf2021-1 induces 100% mortality. The commercial strain exhibited 28% mortality, while the control treatment showed 12%. Statistical analysis reveals a significant difference between treatments (p < 0.01). The Reproductive Efficiency Index (REI) underscores BbSf2021-1is superiority, yielding lower egg weights than other treatments. Regarding the RAI, BbLn2021-1 and BbSf2021-1 show no significant differences but differ significantly from the commercial and control (p < 0.01). These findings suggest that strains isolated and characterized from the natural environment could have potential applications in field trials, serving as a biocontrol alternative for R. microplus ticks.
摘要：
由于其在传播Babesiabigemina中的作用，Rhipicephalusmicroplus构成了重大的经济威胁，B.牛和边缘无性体。化学控制方法，普遍使用，遇到阻力等挑战，高成本,和环境问题。作为一种选择出现，昆虫病原真菌，尤其是白僵菌,为生物防治提供了一条有希望的途径。使用内部转录间隔区(ITS1-5.8-ITS4)区域的分子鉴定确保了准确的物种鉴定。本研究调查了两个白杨菌株，评估它们的分子特征，对R.microplus死亡率的影响，对成年女性的生殖影响。生殖能力倾向指数（RAI）用于评估处理后的tick卵活力，提供这些真菌控制蜱的潜力的见解。结果表明，BbLn2021-1菌株导致96%的死亡率，和BbSf2021-1诱导100%死亡率。商业菌株表现出28%的死亡率，而对照治疗显示12%。统计分析揭示了处理之间的显著差异(p<0.01)。生殖效率指数(REI)强调了BbSf2021-1的优越性，产卵重量比其他处理低。关于RAI,BbLn2021-1和BbSf2021-1没有显着差异，但与商业和对照显着不同（p<0.01）。这些发现表明，从自然环境中分离和表征的菌株可能在田间试验中具有潜在的应用，作为R.microplus蜱的生物防治替代品。
