PDF(Pubmed)
Abstract：
Uncontrolled use of pesticides has caused a dramatic reduction in the number of pollinators, including bees. Studies on the effects of pesticides on bees have reported effects on both metabolic and neurological levels under chronic exposure. In this study, variations in the differential expression of head and thorax-abdomen proteins in Africanized A. mellifera bees treated acutely with sublethal doses of glyphosate and imidacloprid were studied using a proteomic approach. A total of 92 proteins were detected, 49 of which were differentially expressed compared to those in the control group (47 downregulated and 2 upregulated). Protein interaction networks with differential protein expression ratios suggested that acute exposure of A. mellifera to sublethal doses of glyphosate could cause head damage, which is mainly associated with behavior and metabolism. Simultaneously, imidacloprid can cause damage associated with metabolism as well as, neuronal damage, cellular stress, and impairment of the detoxification system. Regarding the thorax-abdomen fractions, glyphosate could lead to cytoskeleton reorganization and a reduction in defense mechanisms, whereas imidacloprid could affect the coordination and impairment of the oxidative stress response.
摘要：
不受控制地使用杀虫剂导致传粉者数量急剧减少,包括蜜蜂。关于农药对蜜蜂影响的研究报告了长期暴露对代谢和神经水平的影响。在这项研究中,使用蛋白质组学方法研究了用亚致死剂量的草甘膦和吡虫啉急性治疗的非洲化A.mellifera蜜蜂中头部和胸腹部蛋白差异表达的变化。共检测到92种蛋白质,其中49个与对照组相比差异表达（47个下调，2个上调）。具有差异蛋白质表达比率的蛋白质相互作用网络表明，A.mellifera急性暴露于亚致死剂量的草甘膦可能导致头部损伤，这主要与行为和新陈代谢有关。同时,吡虫啉可以引起与代谢相关的损伤，神经元损伤，细胞应激，和戒毒系统的损害。关于胸腹部部分，草甘膦可能导致细胞骨架重组和防御机制的减少，吡虫啉可影响氧化应激反应的协调和损害。
