Abstract：
In this study, based on Walker 256 in vitro experiments, CCK-8 assay, clone formation assay, wound healing assay, and flow cytometry were used to detect cell apoptosis and cell cycle. It was found that schisandrin may have significant anti-tumor effects in vitro by inhibiting TGF-β/Smad signaling pathway. In addition, in vivo experiments, immunohistochemistry was used to observe the expression of HIF-1α, VEGF and VEGFR-2 in tumor tissues. It was found that schisandrin could significantly improve the immunosuppression induced by 5-Fu and enhance the antitumor effect of 5-Fu. The mechanism may be related to the inhibition of Wnt-1/β-catenin signaling pathway.
摘要：
在这项研究中,基于Walker256的体外实验，CCK-8测定，克隆形成测定，伤口愈合试验，流式细胞术检测细胞凋亡和细胞周期。研究发现五味子乙素可能通过抑制TGF-β/Smad信号通路在体外具有显著的抗肿瘤作用。此外,体内实验,免疫组化观察HIF-1α的表达,肿瘤组织中VEGF和VEGFR-2。发现五味子甲素能显著改善5-Fu诱导的免疫抑制作用,增强5-Fu的抗肿瘤作用。其机制可能与抑制Wnt-1/β-catenin信号通路有关。
