PDF(Pubmed)
Abstract：
UNASSIGNED: Mobilization of certain immune cells may improve the ability of the immune system to combat tumor cells, but the effect of acute exercise on mobilizing immune cells has been sparsely investigated in cancer patients. Therefore, we examined how acute exercise influences circulating immune cells in breast cancer patients.
UNASSIGNED: Nineteen newly diagnosed breast cancer patients aged 36-68 performed 30 minutes of moderate-intensity exercise with a cycle ergometer. Blood samples were collected at various time points: at rest, at 15 (E15) and 30 minutes (E30) after onset of the exercise, and at 30 and 60 minutes post-exercise. We analyzed several immune cell subsets using flow cytometry.
UNASSIGNED: Acute exercise increased the number of total leukocytes, neutrophils, lymphocytes, monocytes, basophils, total T-cells, CD4+ T-cells, T helper (Th) 2-cells, Th 17-cells, CD8+ T-cells, CD4-CD8- T-cells, CD56+ natural killer (NK) cells, and CD14-CD16+ monocytes. Many of the changes were transient. Proportions of NK-cells and CD8+ T-cells increased, while the proportion of myeloid derived suppressor cells (MDSCs) reduced, and proportion of regulatory T-cells remained unchanged by exercise. Several associations were detected between cell mobilizations and disease state. For instance, tumor size correlated negatively with NK cell mobilization at E15, and progesterone receptor positivity correlated negatively with CD8+ T-cell mobilization.
UNASSIGNED: The findings show that the proportions of CD8+ T-cells and NK cells increased and the proportion of MDSCs proportion decreased in breast cancer patients after 30-minute exercise, suggesting a change in the profile of circulating immune cells towards more cytotoxic/anti-tumorigenic. The mobilization of some immune cells also appears to be related to the disease state.
UNASSIGNED: Nineteen newly diagnosed breast cancer patients aged 36-68 performed 30 minutes of moderate-intensity exercise with a cycle ergometer. Blood samples were collected at various time points: at rest, at 15 (E15) and 30 minutes (E30) after onset of the exercise, and at 30 and 60 minutes post-exercise. We analyzed several immune cell subsets using flow cytometry.
UNASSIGNED: Acute exercise increased the number of total leukocytes, neutrophils, lymphocytes, monocytes, basophils, total T-cells, CD4+ T-cells, T helper (Th) 2-cells, Th 17-cells, CD8+ T-cells, CD4-CD8- T-cells, CD56+ natural killer (NK) cells, and CD14-CD16+ monocytes. Many of the changes were transient. Proportions of NK-cells and CD8+ T-cells increased, while the proportion of myeloid derived suppressor cells (MDSCs) reduced, and proportion of regulatory T-cells remained unchanged by exercise. Several associations were detected between cell mobilizations and disease state. For instance, tumor size correlated negatively with NK cell mobilization at E15, and progesterone receptor positivity correlated negatively with CD8+ T-cell mobilization.
UNASSIGNED: The findings show that the proportions of CD8+ T-cells and NK cells increased and the proportion of MDSCs proportion decreased in breast cancer patients after 30-minute exercise, suggesting a change in the profile of circulating immune cells towards more cytotoxic/anti-tumorigenic. The mobilization of some immune cells also appears to be related to the disease state.
摘要：
■动员某些免疫细胞可能会提高免疫系统对抗肿瘤细胞的能力，但是在癌症患者中，很少研究急性运动对动员免疫细胞的影响。因此,我们研究了急性运动对乳腺癌患者循环免疫细胞的影响.
■19名36-68岁的新诊断乳腺癌患者用周期测功机进行了30分钟的中等强度运动。在不同的时间点收集血样：在休息时，在运动开始后15（E15）和30分钟（E30），运动后30分钟和60分钟。我们使用流式细胞术分析了几种免疫细胞亚群。
■急性运动增加了白细胞总数，中性粒细胞，淋巴细胞，单核细胞,嗜碱性粒细胞,总T细胞，CD4+T细胞，T辅助（Th）2细胞，Th17细胞，CD8+T细胞，CD4-CD8-T细胞,CD56+自然杀伤（NK）细胞，和CD14-CD16+单核细胞。许多变化是短暂的。NK细胞和CD8+T细胞比例增加，而骨髓来源的抑制细胞（MDSCs）的比例降低，通过运动，调节性T细胞的比例保持不变。在细胞动员和疾病状态之间检测到几种关联。例如,E15时肿瘤大小与NK细胞动员呈负相关，孕激素受体阳性与CD8+T细胞动员呈负相关。
■结果表明，30分钟运动后，乳腺癌患者CD8+T细胞和NK细胞比例增加，MDSCs比例降低，提示循环免疫细胞向更具细胞毒性/抗肿瘤性的变化。一些免疫细胞的动员似乎也与疾病状态有关。
■19名36-68岁的新诊断乳腺癌患者用周期测功机进行了30分钟的中等强度运动。在不同的时间点收集血样：在休息时，在运动开始后15（E15）和30分钟（E30），运动后30分钟和60分钟。我们使用流式细胞术分析了几种免疫细胞亚群。
■急性运动增加了白细胞总数，中性粒细胞，淋巴细胞，单核细胞,嗜碱性粒细胞,总T细胞，CD4+T细胞，T辅助（Th）2细胞，Th17细胞，CD8+T细胞，CD4-CD8-T细胞,CD56+自然杀伤（NK）细胞，和CD14-CD16+单核细胞。许多变化是短暂的。NK细胞和CD8+T细胞比例增加，而骨髓来源的抑制细胞（MDSCs）的比例降低，通过运动，调节性T细胞的比例保持不变。在细胞动员和疾病状态之间检测到几种关联。例如,E15时肿瘤大小与NK细胞动员呈负相关，孕激素受体阳性与CD8+T细胞动员呈负相关。
■结果表明，30分钟运动后，乳腺癌患者CD8+T细胞和NK细胞比例增加，MDSCs比例降低，提示循环免疫细胞向更具细胞毒性/抗肿瘤性的变化。一些免疫细胞的动员似乎也与疾病状态有关。
