PDF(Pubmed)
Abstract：
UNASSIGNED: Oligospermia is one of the most common reasons for male infertility which is troubling numerous couples of child-bearing age. This investigation scrutinizes the implications and mechanistic underpinnings of ursolic acid\'s effect on busulfan-induced oligospermia in mouse models.
UNASSIGNED: A singular intraperitoneal injection of busulfan at a dosage of 30 mg/kg induced oligospermia. Two weeks subsequent to this induction, mice were subjected to various dosages of ursolic acid (10, 30, and 50 mg/kg body weight, respectively) on a daily basis for four consecutive weeks. Following this treatment period, a meticulous analysis of epididymal sperm parameters, encompassing concentration and motility, was conducted using a computer-assisted sperm analysis system. The histopathology of the mice testes was performed utilizing hematoxylin and eosin staining, and the cytoskeleton regeneration of the testicular tissues was analyzed via immunofluorescent staining. Serum hormone levels, including testosterone, luteinizing hormone, and follicle-stimulating hormone, as well as reactive oxygen species levels (inclusive of reactive oxygen species and malondialdehyde), were gauged employing specific enzyme-linked immunosorbent assay kits. Differentially expressed genes of testicular mRNA between the oligospermia-induced group and the various ursolic acid treatment groups were identified through RNA sequencing analysis.
UNASSIGNED: The results revealed that a dosage of 50 mg/kg ursolic acid treatment could increase the concentration of epididymal sperm in oligospermia mice, promote the recovery of testicular morphology, regulate hormone levels and ameliorate oxidative damage. The mechanism research results indicated that ursolic acid increased the expression level of genes related to motor proteins in oligospermia mice.
UNASSIGNED: A singular intraperitoneal injection of busulfan at a dosage of 30 mg/kg induced oligospermia. Two weeks subsequent to this induction, mice were subjected to various dosages of ursolic acid (10, 30, and 50 mg/kg body weight, respectively) on a daily basis for four consecutive weeks. Following this treatment period, a meticulous analysis of epididymal sperm parameters, encompassing concentration and motility, was conducted using a computer-assisted sperm analysis system. The histopathology of the mice testes was performed utilizing hematoxylin and eosin staining, and the cytoskeleton regeneration of the testicular tissues was analyzed via immunofluorescent staining. Serum hormone levels, including testosterone, luteinizing hormone, and follicle-stimulating hormone, as well as reactive oxygen species levels (inclusive of reactive oxygen species and malondialdehyde), were gauged employing specific enzyme-linked immunosorbent assay kits. Differentially expressed genes of testicular mRNA between the oligospermia-induced group and the various ursolic acid treatment groups were identified through RNA sequencing analysis.
UNASSIGNED: The results revealed that a dosage of 50 mg/kg ursolic acid treatment could increase the concentration of epididymal sperm in oligospermia mice, promote the recovery of testicular morphology, regulate hormone levels and ameliorate oxidative damage. The mechanism research results indicated that ursolic acid increased the expression level of genes related to motor proteins in oligospermia mice.
摘要：
少精症是男性不育的最常见原因之一，困扰着许多育龄夫妇。这项研究审查了熊果酸对白消安诱导的小鼠模型中的少精子症的影响和机制基础。
■以30mg/kg的剂量单次腹膜内注射白消安诱导的少精子症。诱导后两周，小鼠接受各种剂量的熊果酸（10、30和50mg/kg体重，分别)连续四周每日一次。在这个治疗期之后，细致的附睾精子参数分析，包括浓度和运动性，使用计算机辅助精子分析系统进行。使用苏木精和伊红染色对小鼠睾丸进行组织病理学检查，并通过免疫荧光染色分析睾丸组织的细胞骨架再生。血清激素水平,包括睾丸激素,黄体生成素,和促卵泡激素,以及活性氧水平（包括活性氧和丙二醛），采用特异性酶联免疫吸附测定试剂盒进行测定。通过RNA测序分析鉴定了少精子症诱导组和各个熊果酸治疗组之间睾丸mRNA的差异表达基因。
■结果表明，50mg/kg熊果酸治疗剂量可以增加少精症小鼠附睾精子浓度，促进睾丸形态的恢复，调节激素水平，改善氧化损伤。机制研究结果表明，熊果酸增加了少精子症小鼠运动蛋白相关基因的表达水平。
■以30mg/kg的剂量单次腹膜内注射白消安诱导的少精子症。诱导后两周，小鼠接受各种剂量的熊果酸（10、30和50mg/kg体重，分别)连续四周每日一次。在这个治疗期之后，细致的附睾精子参数分析，包括浓度和运动性，使用计算机辅助精子分析系统进行。使用苏木精和伊红染色对小鼠睾丸进行组织病理学检查，并通过免疫荧光染色分析睾丸组织的细胞骨架再生。血清激素水平,包括睾丸激素,黄体生成素,和促卵泡激素,以及活性氧水平（包括活性氧和丙二醛），采用特异性酶联免疫吸附测定试剂盒进行测定。通过RNA测序分析鉴定了少精子症诱导组和各个熊果酸治疗组之间睾丸mRNA的差异表达基因。
■结果表明，50mg/kg熊果酸治疗剂量可以增加少精症小鼠附睾精子浓度，促进睾丸形态的恢复，调节激素水平，改善氧化损伤。机制研究结果表明，熊果酸增加了少精子症小鼠运动蛋白相关基因的表达水平。
