PDF(Pubmed)
Abstract：
OBJECTIVE: Use of numerous medications such as tyrosine kinase inhibitors (sunitinib), monoclonal antibodies (bevacizumab), fusion proteins (aflibercept), mTOR inhibitors (everolimus), radiopharmaceuticals (radium 223), selective estrogen receptor modulators (raloxifene), and immunosuppressants (methotrexate and corticosteroids) has been reported to be a risk factor for development of medication-related osteonecrosis of the jaws till date. This study aimed to evaluate the preventive effect of low-level laser therapy (LLLT) and gaseous ozone on the onset of MRONJ following tooth extraction.
METHODS: A total of 40 male Wistar rats were randomly allocated into 4 groups of 10 rats each. The groups laser (L), ozone (O), and control (C) received weekly intraperitoneal injections of zoledronic acid (0.06 mg/kg), while group sham (S) received saline solution for 4 weeks. After the 4th injection, all subjects underwent mandibular first molar extraction and adjunctive laser or ozone was applied according to the groups. All the rats were sacrificed at 4 postoperative weeks for comparative histomorphometric evaluation of bone healing in extraction sites.
RESULTS: Laser and ozone groups demonstrated significantly higher bone formation compared to control group (p < 0.05), while no significant difference was found between laser and ozone groups (p = 1.00). Furthermore, the greatest bone formation was observed with the sham group (p < 0.05).
CONCLUSIONS: Findings of the current study support that adjunctive LLLT and ozone therapy following tooth extraction may help prevent MRONJ and improve bone healing in subjects under zoledronic acid therapy.
CONCLUSIONS: Since the introduction in 2003, great effort has been devoted to developing a certain management protocol for MRONJ. Several publications have appeared in recent years documenting promising results of adjunctive LLLT and ozone application in treatment of MRONJ. However, experimental data are limited on this regard and the present study, for the first time, aimed to evaluate and compare the effects of LLLT and ozone in prevention of MRONJ.
METHODS: A total of 40 male Wistar rats were randomly allocated into 4 groups of 10 rats each. The groups laser (L), ozone (O), and control (C) received weekly intraperitoneal injections of zoledronic acid (0.06 mg/kg), while group sham (S) received saline solution for 4 weeks. After the 4th injection, all subjects underwent mandibular first molar extraction and adjunctive laser or ozone was applied according to the groups. All the rats were sacrificed at 4 postoperative weeks for comparative histomorphometric evaluation of bone healing in extraction sites.
RESULTS: Laser and ozone groups demonstrated significantly higher bone formation compared to control group (p < 0.05), while no significant difference was found between laser and ozone groups (p = 1.00). Furthermore, the greatest bone formation was observed with the sham group (p < 0.05).
CONCLUSIONS: Findings of the current study support that adjunctive LLLT and ozone therapy following tooth extraction may help prevent MRONJ and improve bone healing in subjects under zoledronic acid therapy.
CONCLUSIONS: Since the introduction in 2003, great effort has been devoted to developing a certain management protocol for MRONJ. Several publications have appeared in recent years documenting promising results of adjunctive LLLT and ozone application in treatment of MRONJ. However, experimental data are limited on this regard and the present study, for the first time, aimed to evaluate and compare the effects of LLLT and ozone in prevention of MRONJ.
摘要：
目的：使用多种药物，如酪氨酸激酶抑制剂（舒尼替尼），单克隆抗体（贝伐单抗），融合蛋白（阿柏西普），mTOR抑制剂（依维莫司），放射性药物（镭223），选择性雌激素受体调节剂（雷洛昔芬），据报道，免疫抑制剂（甲氨蝶呤和皮质类固醇）是与药物相关的颌骨坏死发展的危险因素。这项研究旨在评估低水平激光治疗（LLLT）和气态臭氧对拔牙后MRONJ发作的预防作用。
方法：将40只雄性Wistar大鼠随机分为4组，每组10只。组激光（L），臭氧(O),和对照组（C）每周腹膜内注射唑来膦酸（0.06mg/kg），而sham组（S）接受生理盐水4周。第4次注射后,所有受试者均接受下颌第一磨牙拔除术，并根据各组应用辅助激光或臭氧。所有大鼠在术后4周时处死,以比较组织形态学评价拔牙部位的骨愈合。
结果：与对照组相比，激光和臭氧组显示出较高的骨形成（p<0.05），而激光组和臭氧组之间没有发现显着差异（p=1.00）。此外，假手术组骨形成最大(p<0.05)。
结论：当前研究的结果支持拔牙后辅助LLLT和臭氧治疗可能有助于预防MRONJ并改善唑来膦酸治疗受试者的骨愈合。
结论：自2003年推出以来，人们一直在努力为MRONJ开发某种管理协议。近年来已经出现了一些出版物，这些出版物记录了在MRONJ治疗中辅助LLLT和臭氧应用的有希望的结果。然而,这方面的实验数据有限，目前的研究，第一次,旨在评估和比较LLLT和臭氧预防MRONJ的效果。
方法：将40只雄性Wistar大鼠随机分为4组，每组10只。组激光（L），臭氧(O),和对照组（C）每周腹膜内注射唑来膦酸（0.06mg/kg），而sham组（S）接受生理盐水4周。第4次注射后,所有受试者均接受下颌第一磨牙拔除术，并根据各组应用辅助激光或臭氧。所有大鼠在术后4周时处死,以比较组织形态学评价拔牙部位的骨愈合。
结果：与对照组相比，激光和臭氧组显示出较高的骨形成（p<0.05），而激光组和臭氧组之间没有发现显着差异（p=1.00）。此外，假手术组骨形成最大(p<0.05)。
结论：当前研究的结果支持拔牙后辅助LLLT和臭氧治疗可能有助于预防MRONJ并改善唑来膦酸治疗受试者的骨愈合。
结论：自2003年推出以来，人们一直在努力为MRONJ开发某种管理协议。近年来已经出现了一些出版物，这些出版物记录了在MRONJ治疗中辅助LLLT和臭氧应用的有希望的结果。然而,这方面的实验数据有限，目前的研究，第一次,旨在评估和比较LLLT和臭氧预防MRONJ的效果。
