PDF(Pubmed)
Abstract：
Although bone dehiscence may occur during orthodontic tooth movement into the narrow alveolar ridge, a non-invasive prevention method is yet to be fully established. We show for the first time prevention of bone dehiscence associated with orthodontic tooth movement by prophylactic injection of bone anabolic agents in mice. In this study, we established a bone dehiscence mouse model by applying force application and used the granular type of scaffold materials encapsulated with bone morphogenetic protein (BMP)-2 and OP3-4, the receptor activator of NF-κB ligand (RANKL)-binding peptide, for the prophylactic injection to the alveolar bone. In vivo micro-computed tomography revealed bone dehiscence with decreased buccal alveolar bone thickness and height after force application, whereas no bone dehiscence was observed with the prophylactic injection after force application, and alveolar bone thickness and height were kept at similar levels as those in the control group. Bone histomorphometry analyses revealed that both bone formation and resorption parameters were significantly higher in the injection with force application group than in the force application without the prophylactic injection group. These findings suggest that the prophylactic local delivery of bone anabolic reagents can prevent bone dehiscence with increased bone remodelling activity.
摘要：
尽管在正畸牙齿移动到狭窄的牙槽脊期间可能会发生骨裂开，非侵入性预防方法尚未完全建立。我们首次展示了通过在小鼠中预防性注射骨合成代谢剂预防与正畸牙齿移动相关的骨开裂。在这项研究中,我们通过施加力建立了骨裂开小鼠模型，并使用了包裹有骨形态发生蛋白（BMP）-2和OP3-4的颗粒型支架材料，NF-κB配体（RANKL）结合肽的受体激活剂，用于牙槽骨的预防性注射。体内显微计算机断层扫描显示骨裂开，用力后颊牙槽骨厚度和高度降低，而在施力后，预防性注射没有观察到骨裂开，牙槽骨厚度和高度保持在与对照组相似的水平。骨组织形态计量学分析显示，带力注射组的骨形成和吸收参数均显着高于不带力注射组。这些发现表明，预防性局部递送骨合成代谢试剂可以防止骨开裂，并增加骨重塑活性。
