Abstract：
This study explored the extracellular metabolomic responses of three different Salmonella enterica serotype Typhimurium (S. Typhimurium) strains-ATCC 13311 (STy1), NCCP 16964 (STy4), and NCCP 16958 (STy8)-cultured at refrigeration temperatures. The objective was to identify the survival mechanisms of S. Typhimurium under cold stress by analyzing variations in their metabolomic profiles. Qualitative and quantitative assessments identified significant metabolite alterations on day 6, marking a critical inflection point. Key metabolites such as trehalose, proline, glycerol, and tryptophan were notably upregulated in response to cold stress. Through multivariate analyses, the strains were distinguished using three metabolites-4-aminobutyrate, ethanol, and uridine-as potential biomarkers, underscoring distinct metabolic responses to refrigeration. Specifically, STy1 exhibited unique adaptive capabilities through enhanced metabolism of betaine and 4-aminobutyrate. These findings highlight the variability in adaptive strategies among S. Typhimurium strains, suggesting that certain strains may possess more robust metabolic pathways for enhancing survival in refrigerated conditions.
摘要：
本研究探索了三种不同血清型鼠伤寒沙门氏菌（S.鼠伤寒）菌株-ATCC13311（STy1），NCCP16964(STy4),和NCCP16958（STy8）在冷藏温度下培养。目的是通过分析其代谢组学谱的变化来确定鼠伤寒沙门氏菌在冷应激下的存活机制。定性和定量评估在第6天确定了显著的代谢物改变,标志着关键的拐点。关键代谢物如海藻糖，脯氨酸,甘油，和色氨酸在冷应激时显著上调。通过多变量分析，使用三种代谢物-4-氨基丁酸来区分菌株，乙醇,和尿苷-作为潜在的生物标志物，强调对冷藏的不同代谢反应。具体来说,STy1通过增强甜菜碱和4-氨基丁酸的代谢表现出独特的适应能力。这些发现强调了鼠伤寒沙门氏菌菌株之间适应性策略的变异性，这表明某些菌株可能具有更强大的代谢途径来增强在冷藏条件下的存活。
