Abstract：
This study explores the implementation of the One Sample Strategy (OSS), a co-regulation program aimed at managing mycotoxin risk in Texas maize. Fumonisin-contaminated cereals and oilseeds that contain greater than 5 mg kg-1 of the toxin (B1, B2, and B3) are a risk for equids and rabbits, and levels greater than 60 mg kg-1 are a risk to ruminants. The OSS, previously successful in managing aflatoxin risk in Texas maize, was evaluated for its effectiveness in handling fumonisin risk in maize, specifically as it relates to ruminants. In 2017, 25 analysts across seven firms qualified to participate in the program. To ensure greater accuracy in testing, working control samples were provided to the participating OSS firms with the requirement that their results fall within +/- 20% of the target concentration. Ninety-four percent of the working controls met this specification. The capability to grind maize to the OSS prescribed particle size was met by 100% of participants. To verify testing accuracy, file samples collected from each OSS firm were analysed by UPLC-MS/MS. The 177 fumonisin verification samples analysed by Office of the Texas State Chemist (OTSC) were correlated (r = 0.93) with co-regulation laboratories. Results were plotted in an operating curve to depict type I and type II errors. Error analysis revealed a type I error rate of 13% and type II error rate of 2% for the 5 mg kg-1 guidance level, and 6% and 8%, respectively, for the 60 mg kg-1 guidance level. For 2017, 994 official reports of analysis for fumonisin in whole maize in the Texas High Plains were issued by the seven laboratories that employed 25 OTSC-credentialed analysts. The OSS co-regulation program, supported by a quality systems approach and government regulations, has proven effective in managing fumonisin risk in Texas maize, enhancing both market confidence and livestock safety.
摘要：
本研究探讨了一个样本策略(OSS)的实施，旨在管理德克萨斯州玉米霉菌毒素风险的共同监管计划。含有超过5mgkg-1毒素（B1，B2和B3）的受伏马菌素污染的谷物和油籽对马科动物和兔子有风险，和水平大于60mgkg-1是反刍动物的风险。OSS,以前成功地管理了德克萨斯州玉米中的黄曲霉毒素风险，对其在处理玉米伏马菌素风险方面的有效性进行了评估，特别是与反刍动物有关。2017年，七家公司的25名分析师有资格参与该计划。为了确保测试的准确性，工作对照样品提供给参与的OSS公司，要求其结果落在目标浓度的+/-20%以内。百分之九十四的工作控制符合该规范。100%的参与者达到了将玉米研磨至OSS规定的粒度的能力。为了验证测试的准确性，UPLC-MS/MS分析了从每家OSS公司收集的文件样本。得克萨斯州化学家办公室（OTSC）分析的177个伏马菌素验证样品与共同监管实验室相关（r=0.93）。将结果绘制在操作曲线中以描绘I型和II型误差。误差分析显示，对于5mgkg-1的指导水平，I型错误率为13％，II型错误率为2％，6%和8%，分别,对于60mgkg-1的指导水平。2017年，七个实验室发布了994份关于得克萨斯州高平原地区整个玉米中伏马菌素的官方分析报告，这些实验室雇用了25名OTSC认证的分析师。OSS共同监管计划，在质量体系方法和政府法规的支持下，已经证明在管理德州玉米伏马菌素风险方面是有效的，增强市场信心和牲畜安全。
