Abstract：
BACKGROUND: Discussion of the benefits of moderate alcohol consumption is ongoing. Broadly, research focusing on ethanol consumption tends to report no benefits. However, studies that distinguish between different types of alcoholic beverages, particularly beers, often reveal positive effects. The present study evaluated the genotoxic and mutagenic effects of moderate chronic consumption of India Pale Ale (IPA) craft beer. Sixty-four adult male Swiss mice were used and divided into control and treatment groups receiving water, IPA beer with 55.23 g of ethanol per liter of beer, aqueous solution with 55.23 g of ethanol per liter, and hop infusion ad libitum for 30 days. After this period, the animals were genetically evaluated with a comet assay. For the ex vivo comet assay, blood was collected and exposed to hydrogen peroxide (H2O2). For the in vivo assay, the alkylating agent cyclophosphamide (CP) was administered to the groups after blood collection and sacrificed after 24 h. Brain, liver, and heart tissues were analyzed. Bone marrow was collected and submitted to the micronucleus test.
RESULTS: The groups treated with IPA beer, ethanol, and hops did not show genotoxic and mutagenic action in the blood, brain, heart, or liver. The antigenotoxic action of IPA beer and hops was observed in both in vivo and ex vivo models, showing a similar reduction in DNA damage caused by CP. There was no significant difference between the groups with regard to the formation of micronuclei by CP.
CONCLUSIONS: Moderate chronic consumption of IPA beer and hops infusion showed antigenotoxic effects in mice but no antimutagenic action. © 2024 Society of Chemical Industry.
RESULTS: The groups treated with IPA beer, ethanol, and hops did not show genotoxic and mutagenic action in the blood, brain, heart, or liver. The antigenotoxic action of IPA beer and hops was observed in both in vivo and ex vivo models, showing a similar reduction in DNA damage caused by CP. There was no significant difference between the groups with regard to the formation of micronuclei by CP.
CONCLUSIONS: Moderate chronic consumption of IPA beer and hops infusion showed antigenotoxic effects in mice but no antimutagenic action. © 2024 Society of Chemical Industry.
摘要：
背景：关于适度饮酒的益处的讨论正在进行中。广义上,专注于乙醇消费的研究往往没有任何好处。然而,区分不同类型酒精饮料的研究，尤其是啤酒,经常显示出积极的影响。本研究评估了适度慢性食用印度淡啤酒（IPA）精酿啤酒的遗传毒性和诱变作用。使用64只成年雄性瑞士小鼠，并分为对照组和接受水的治疗组，IPA啤酒，每升啤酒含55.23克乙醇，每升含55.23克乙醇的水溶液，并随意跳跃输注30天。在这段时间之后,用彗星试验对动物进行遗传评估。对于离体彗星测定，收集血液并暴露于过氧化氢（H2O2）。对于体内测定，在采血后将烷化剂环磷酰胺（CP）给予各组，并在24小时后处死。肝脏,和心脏组织进行了分析。收集骨髓并进行微核试验。
结果：用IPA啤酒治疗的组，乙醇,啤酒花在血液中没有遗传毒性和诱变作用，大脑,心,或者肝脏。在体内和离体模型中都观察到IPA啤酒和啤酒花的抗基因毒性作用。显示由CP引起的DNA损伤的类似减少。在CP形成微核方面，两组之间没有显着差异。
结论：中度长期饮用IPA啤酒和啤酒花对小鼠有抗遗传毒性作用，但没有抗诱变作用。©2024化学工业学会。
结果：用IPA啤酒治疗的组，乙醇,啤酒花在血液中没有遗传毒性和诱变作用，大脑,心,或者肝脏。在体内和离体模型中都观察到IPA啤酒和啤酒花的抗基因毒性作用。显示由CP引起的DNA损伤的类似减少。在CP形成微核方面，两组之间没有显着差异。
结论：中度长期饮用IPA啤酒和啤酒花对小鼠有抗遗传毒性作用，但没有抗诱变作用。©2024化学工业学会。
