Abstract：
BACKGROUND: Plants have been used for ages in traditional medicine, and it is exciting to perceive how recent research has recognized the bioactive compounds liable for their beneficial effects. Green synthesis of metal nanoparticles is a hastily emergent research area in nanotechnology. This study describes the synthesis of silver nanoparticles (AgNPs) using Coriandrum sativum and Murraya koenigii leaf extract and its thrombolytic activity.
OBJECTIVE: The aim of the study was to determine the clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles.
METHODS: Leaves of Coriandrum sativum and Murraya koenigii were collected. Methanolic extraction of the plant sample was done through a Soxhlet extractor. The methanolic extract obtained from both the leaves was subjected to GC-MS analysis. The synthesized NPs from leaf extracts were monitored for analysis, where the typical X-ray diffraction pattern and its diffraction peaks were identified. 3D image of the NPs was analysed by Atomic Force Microscopy. The surface charge of nanoparticles was identified by Zeta potential. The Clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles were analysed by the modified Holmstorm method.
RESULTS: The thrombolytic property of the methanolic extract of plants Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 45.99% activity, and Murraya koenigii extract with 66.56% activity. The nanoparticles (Nps) from Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 58.29% activity, and NPs from Murraya koenigii with 54.04% activity. Coriandrum sativum in GC-MS exhibited 3 peaks, whereas Murraya koenigii extract showed five peaks with notable bioactive compounds.
CONCLUSIONS: These NPs were further used for biomedical applications after being fixed by an organic encapsulation agent. The present research reveals the usefulness of Coriandrum sativum and Murraya koenigii for the environmentally friendly manufacture of silver nanoparticles.
OBJECTIVE: The aim of the study was to determine the clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles.
METHODS: Leaves of Coriandrum sativum and Murraya koenigii were collected. Methanolic extraction of the plant sample was done through a Soxhlet extractor. The methanolic extract obtained from both the leaves was subjected to GC-MS analysis. The synthesized NPs from leaf extracts were monitored for analysis, where the typical X-ray diffraction pattern and its diffraction peaks were identified. 3D image of the NPs was analysed by Atomic Force Microscopy. The surface charge of nanoparticles was identified by Zeta potential. The Clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles were analysed by the modified Holmstorm method.
RESULTS: The thrombolytic property of the methanolic extract of plants Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 45.99% activity, and Murraya koenigii extract with 66.56% activity. The nanoparticles (Nps) from Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 58.29% activity, and NPs from Murraya koenigii with 54.04% activity. Coriandrum sativum in GC-MS exhibited 3 peaks, whereas Murraya koenigii extract showed five peaks with notable bioactive compounds.
CONCLUSIONS: These NPs were further used for biomedical applications after being fixed by an organic encapsulation agent. The present research reveals the usefulness of Coriandrum sativum and Murraya koenigii for the environmentally friendly manufacture of silver nanoparticles.
摘要：
背景：植物已经在传统医学中使用了很长时间，令人兴奋的是，最近的研究如何认识到生物活性化合物对其有益的作用。金属纳米粒子的绿色合成是纳米技术中一个草率出现的研究领域。这项研究描述了使用Coriandrumsativum和Murrayakoenigii叶提取物合成银纳米颗粒（AgNPs）及其溶栓活性。
目的：研究的目的是确定Coriandrumsativum和Murrayakoenigii合成的银纳米颗粒的凝块溶解活性。
方法：收集金花和Murrayakoenigii的叶子。通过索氏提取器进行植物样品的甲醇提取。对从两片叶子获得的甲醇提取物进行GC-MS分析。监测来自叶提取物的合成NP进行分析，其中确定了典型的X射线衍射图及其衍射峰。通过原子力显微镜分析NP的3D图像。纳米颗粒的表面电荷由Zeta电位鉴定。通过改进的Holmstorm方法分析了Coriandrumsativum和Murrayakoenigii合成的银纳米颗粒的凝集裂解活性。
结果：植物Coriandrumsativum的甲醇提取物的溶血栓特性显示，凝块溶解活性为2.5mg/mL，活性为45.99％，和Murrayakoenigii提取物，活性为66.56%。来自Coriandrumsativum的纳米颗粒（Nps）在2.5mg/mL时显示出凝块溶解活性，活性为58.29％，和来自Murrayakoenigii的NPs，活性为54.04%。香茅在GC-MS中出现3个峰,而Murrayakoenigii提取物显示出五个具有显着生物活性化合物的峰。
结论:这些NP在被有机包封剂固定后进一步用于生物医学应用。本研究揭示了Coriandrumsativum和Murrayakoenigii对环境友好的银纳米颗粒制造的有用性。
目的：研究的目的是确定Coriandrumsativum和Murrayakoenigii合成的银纳米颗粒的凝块溶解活性。
方法：收集金花和Murrayakoenigii的叶子。通过索氏提取器进行植物样品的甲醇提取。对从两片叶子获得的甲醇提取物进行GC-MS分析。监测来自叶提取物的合成NP进行分析，其中确定了典型的X射线衍射图及其衍射峰。通过原子力显微镜分析NP的3D图像。纳米颗粒的表面电荷由Zeta电位鉴定。通过改进的Holmstorm方法分析了Coriandrumsativum和Murrayakoenigii合成的银纳米颗粒的凝集裂解活性。
结果：植物Coriandrumsativum的甲醇提取物的溶血栓特性显示，凝块溶解活性为2.5mg/mL，活性为45.99％，和Murrayakoenigii提取物，活性为66.56%。来自Coriandrumsativum的纳米颗粒（Nps）在2.5mg/mL时显示出凝块溶解活性，活性为58.29％，和来自Murrayakoenigii的NPs，活性为54.04%。香茅在GC-MS中出现3个峰,而Murrayakoenigii提取物显示出五个具有显着生物活性化合物的峰。
结论:这些NP在被有机包封剂固定后进一步用于生物医学应用。本研究揭示了Coriandrumsativum和Murrayakoenigii对环境友好的银纳米颗粒制造的有用性。
