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Abstract：
Aim The aim of this study was to assess the physicochemical characterization and mineralization of nanofibrous scaffold incorporated with nanohydroxyapatite (nHA) and aspartic acid (Asp) for dental mineralization. Methodology Three nanofibrous scaffolds were prepared, namely polycaprolactone (PCL), PCL with nHA, and PCL with nHA and Asp. Each scaffold was prepared separately by electrospinning. The physicochemical characterization of the surface of the nanofibrous scaffold was imaged using a scanning electron microscope (SEM), energy dispersive X-ray Analysis (EDX), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). In vitro mineralization studies were performed by immersing the sample in simulated body fluid (SBF) for 7, 14, and 21 days. The surface of the samples was observed under SEM with EDX. Results SEM analysis of PCL/nHA/Asp revealed that the nanofibers were bead-free, smooth, randomly oriented, and loaded with Asp. The EDX spectra of PCL/nHA/Asp composite nanofibrous scaffold revealed broad peaks and corresponded to the amorphous form, while the sharp peaks corresponded to the specific crystalline structure of nHA. FTIR analysis showed specific functional groups corresponding to PCL, nHA, and Asp. The scaffolds incorporated with Asp exhibited higher mineralization potential with an apatite-like crystal formation, which increased with an increase in the duration of immersion in SBF. Conclusion Physiochemical characterization demonstrated the incorporation of PCL/nHA/Asp in the electrospun nanofibrous scaffold. The mineralization analysis revealed that the presence of Asp enhanced the mineralization when compared with the PCL and PCL/nHA. PCL/nHA/Asp incorporated in scaffold can be a promising material for dental mineralization.
摘要：
目的本研究的目的是评估与纳米羟基磷灰石（nHA）和天冬氨酸（Asp）结合用于牙科矿化的纳米纤维支架的物理化学表征和矿化。方法制备三种纳米纤维支架,即聚己内酯(PCL)，带nHA的PCL,以及具有nHA和Asp的PCL。通过静电纺丝分别制备每个支架。使用扫描电子显微镜（SEM）对纳米纤维支架表面的物理化学表征进行了成像，能量色散X射线分析（EDX），X射线衍射(XRD)和傅里叶变换红外光谱(FTIR)。通过将样品浸入模拟体液（SBF）中7、14和21天进行体外矿化研究。在具有EDX的SEM下观察样品的表面。结果对PCL/nHA/Asp的SEM分析显示，纳米纤维无珠，光滑,随机定向，装满了Asp.PCL/nHA/Asp复合纳米纤维支架的EDX光谱显示宽峰，对应于无定形形式，而尖锐的峰对应于nHA的特定晶体结构。FTIR分析显示对应于PCL的特定官能团，nHA,Asp。与Asp结合的scafiOds表现出更高的矿化潜力，并形成磷灰石状晶体，随着浸入SBF的持续时间的增加而增加。结论理化表征表明PCL/nHA/Asp在电纺纳米纤维支架中的掺入。矿化分析表明，与PCL和PCL/nHA相比，Asp的存在增强了矿化作用。在支架中掺入PCL/nHA/Asp可以是用于牙齿矿化的有前途的材料。
