PDF(Pubmed)
Abstract：
UNASSIGNED: In order to detect early gastric cancer (EGC), this research sought to assess the diagnostic utility of magnifying endoscopy (ME) as well as the significance of mucin phenotype and microvessel features.
UNASSIGNED: 402 individuals with an EGC diagnosis underwent endoscopic submucosal dissection (ESD) at the Department of ME between 2012 and 2020. After adjusting for image distortion, high-magnification endoscopic pictures were taken and examined to find microvessels in the area of interest. The microvessel density was measured as counts per square millimeter (counts/mm2) after segmentation, and the vascular bed\'s size was computed as a percentage of the area of interest. To identify certain properties of the microvessels, such as end-points, crossing points, branching sites, and connection points, further processing was done using skeletonized pixels.
UNASSIGNED: According to the research, undifferentiated tumors often lacked the MS pattern and showed an oval and tubular microsurface (MS) pattern, but differentiated EGC tumors usually lacked the MS pattern and presented a corkscrew MV pattern. Submucosal invasion was shown to be more strongly associated with the destructive MS pattern in differentiated tumors as opposed to undifferentiated tumors. While lesions with a corkscrew MV pattern and an antrum or body MS pattern revealed greater MUC5AC expression, lesions with a loop MV pattern indicated higher MUC2 expression. Furthermore, CD10 expression was higher in lesions with a papillary pattern and an antrum or body MS pattern.
UNASSIGNED: These results imply that evaluating mucin phenotype and microvessel features in conjunction with magnifying endoscopy (ME) may be a useful diagnostic strategy for early gastric cancer (EGC) detection. Nevertheless, further investigation is required to confirm these findings and identify the best course of action for EGC diagnosis.
UNASSIGNED: 402 individuals with an EGC diagnosis underwent endoscopic submucosal dissection (ESD) at the Department of ME between 2012 and 2020. After adjusting for image distortion, high-magnification endoscopic pictures were taken and examined to find microvessels in the area of interest. The microvessel density was measured as counts per square millimeter (counts/mm2) after segmentation, and the vascular bed\'s size was computed as a percentage of the area of interest. To identify certain properties of the microvessels, such as end-points, crossing points, branching sites, and connection points, further processing was done using skeletonized pixels.
UNASSIGNED: According to the research, undifferentiated tumors often lacked the MS pattern and showed an oval and tubular microsurface (MS) pattern, but differentiated EGC tumors usually lacked the MS pattern and presented a corkscrew MV pattern. Submucosal invasion was shown to be more strongly associated with the destructive MS pattern in differentiated tumors as opposed to undifferentiated tumors. While lesions with a corkscrew MV pattern and an antrum or body MS pattern revealed greater MUC5AC expression, lesions with a loop MV pattern indicated higher MUC2 expression. Furthermore, CD10 expression was higher in lesions with a papillary pattern and an antrum or body MS pattern.
UNASSIGNED: These results imply that evaluating mucin phenotype and microvessel features in conjunction with magnifying endoscopy (ME) may be a useful diagnostic strategy for early gastric cancer (EGC) detection. Nevertheless, further investigation is required to confirm these findings and identify the best course of action for EGC diagnosis.
摘要：
■为了检测早期胃癌（EGC），本研究旨在评估放大内镜(ME)的诊断效用以及粘蛋白表型和微血管特征的意义.
■402名诊断为EGC的患者在2012年至2020年期间在ME部门接受了内镜黏膜下剥离术（ESD）。调整图像失真后，我们拍摄了高倍率内窥镜照片并进行了检查,以发现感兴趣区域的微血管.分割后，以每平方毫米的计数（计数/mm2）测量微血管密度，血管床的大小计算为感兴趣面积的百分比。为了识别微血管的某些特性，如终点，交叉点,分支位点，和连接点，使用骨架化像素进行进一步处理。
■根据研究，未分化肿瘤通常缺乏MS模式，并显示椭圆形和管状微表面（MS）模式，但是分化的EGC肿瘤通常缺乏MS模式，并呈现开瓶器MV模式。与未分化肿瘤相比，粘膜下浸润与分化肿瘤的破坏性MS模式更密切相关。虽然具有开瓶器MV模式和胃窦或身体MS模式的病变显示出更高的MUC5AC表达，具有环状MV模式的病变表明MUC2表达较高。此外，在具有乳头状模式和窦或身体MS模式的病变中，CD10表达更高。
■这些结果表明，结合放大内镜（ME）评估粘蛋白表型和微血管特征可能是早期胃癌（EGC）检测的有用诊断策略。然而，需要进一步的研究来确认这些发现,并确定EGC诊断的最佳措施.
■402名诊断为EGC的患者在2012年至2020年期间在ME部门接受了内镜黏膜下剥离术（ESD）。调整图像失真后，我们拍摄了高倍率内窥镜照片并进行了检查,以发现感兴趣区域的微血管.分割后，以每平方毫米的计数（计数/mm2）测量微血管密度，血管床的大小计算为感兴趣面积的百分比。为了识别微血管的某些特性，如终点，交叉点,分支位点，和连接点，使用骨架化像素进行进一步处理。
■根据研究，未分化肿瘤通常缺乏MS模式，并显示椭圆形和管状微表面（MS）模式，但是分化的EGC肿瘤通常缺乏MS模式，并呈现开瓶器MV模式。与未分化肿瘤相比，粘膜下浸润与分化肿瘤的破坏性MS模式更密切相关。虽然具有开瓶器MV模式和胃窦或身体MS模式的病变显示出更高的MUC5AC表达，具有环状MV模式的病变表明MUC2表达较高。此外，在具有乳头状模式和窦或身体MS模式的病变中，CD10表达更高。
■这些结果表明，结合放大内镜（ME）评估粘蛋白表型和微血管特征可能是早期胃癌（EGC）检测的有用诊断策略。然而，需要进一步的研究来确认这些发现,并确定EGC诊断的最佳措施.
