PDF(Pubmed)
Abstract：
Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. Expansion microscopy has revolutionised this topic. Here we present and compare two protocols of ultrastructure expansion microscopy that allow for 4.5-fold mostly isotropic expansion and the use of antibodies, metabolic labelling, and DNA stains to demarcate individual regions such as the endoplasmic reticulum, the nuclei, the peripheral endocytic compartments as well as the ventral disc and the cytoskeleton in Giardia lamblia. We present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs in this important protozoan model organism.
摘要：
了解细胞超微结构与微观分辨率以及在该分辨率下识别单个成分的能力密切相关。扩展显微镜彻底改变了这个话题。在这里，我们提出并比较了两种超微结构扩展显微镜的方案，这些方案允许4.5倍的各向同性扩展和抗体的使用，代谢标记，和DNA染色来划分各个区域，如内质网，原子核，蓝氏贾第鞭毛虫的外周内吞区室以及腹盘和细胞骨架。我们提出了一个优化的，缩短，和模块化协议，可以迅速调整，以满足研究人员在这个重要的原生动物模型生物的需要。
