Abstract：
DNA nanostructures have been utilized to study biological mechanical processes and construct artificial nanosystems. Many application scenarios necessitate nanodevices able to robustly generate large single molecular forces. However, most existing dynamic DNA nanostructures are triggered by probabilistic hybridization reactions between spatially separated DNA strands, which only non-deterministically generate relatively small compression forces (≈0.4 piconewtons (pN)). Here, an intercalator-triggered dynamic DNA origami nanostructure is developed, where large amounts of local binding reactions between intercalators and the nanostructure collectively lead to the robust generation of relatively large compression forces (≈11.2 pN). Biomolecular loads with different stiffnesses, 3, 4, and 6-helix DNA bundles are efficiently bent by the compression forces. This work provides a robust and powerful force-generation tool for building highly chemo-mechanically coupled molecular machines in synthetic nanosystems.
摘要：
DNA纳米结构已用于研究生物机械过程并构建人造纳米系统。许多应用场景需要能够稳健地产生大的单分子力的纳米器件。然而,大多数现有的动态DNA纳米结构是由空间分离的DNA链之间的概率杂交反应触发的，仅非确定性地产生相对较小的压缩力（≈0.4皮牛顿（pN））。这里,开发了嵌入剂触发的动态DNA折纸纳米结构，其中嵌入剂和纳米结构之间的大量局部结合反应共同导致相对较大的压缩力（≈11.2pN）的强大产生。具有不同刚度的生物分子负载，3、4和6螺旋DNA束被压缩力有效地弯曲。这项工作提供了一个强大而强大的力生成工具，用于在合成纳米系统中构建高度化学机械耦合的分子机器。
