Abstract：
Strains of the bacterial pathogen Xylella fastidiosa subspecies multiplex (Xfm) and pauca (Xfp) isolated from symptomatic almond and olive plants in Spain and Italy were used in this study. Because of the risk of host jump and considering the importance of southern highbush blueberry production in Spain, we tested a small set of these strains for their potential to infect and cause disease symptoms in blueberries under greenhouse experiments. Xfm IVIA5901 (isolated from almonds in Alicante, Spain) caused symptoms similar to those caused by Xfm AlmaEm3 (isolated from blueberries in Georgia, U.S.A., and used as a reference strain capable of inducing severe symptoms in blueberry). Nevertheless, bacterial populations of Xfm IVIA5901 in planta were significantly lower than those of Xfm AlmaEm3. Xfm ESVL (isolated from almonds, Alicante, Spain) and Xfp XYL1961/18 (isolated from olives, Ibiza Island, Spain) caused limited symptoms, while Xfm XYL466/19 (isolated from wild olives, Mallorca Island, Spain) and Xfm XF3348 (isolated from almonds, Mallorca Island, Spain) and Xfp De Donno (isolated from olives, Puglia, Italy, and representative of the devastating olive quick decline syndrome) did not cause symptoms nor colonize blueberries. This study suggests that certain strains already found in Europe could infect blueberry if conditions conducive for a host jump in this region are met, such as proximity of blueberries to other infected hosts and presence of insect vectors that feed on these crops. Surveys on the presence of X. fastidiosa in blueberries in Spain and other European countries are needed to anticipate possible issues.
摘要：
本研究使用了从西班牙和意大利的有症状的杏仁和橄榄植物中分离出的细菌病原体Xylellafastidiosa亚种（Xfm）和pauca（Xfp）的菌株。由于主机跳跃的风险，并考虑到西班牙南部高灌木丛蓝莓生产的重要性，我们在温室实验中测试了一小部分这些菌株感染蓝莓并引起疾病症状的潜力。XfmIVIA5901（从阿利坎特的杏仁中分离出来，西班牙）引起的症状类似于XfmAlmaEM3（从格鲁吉亚的蓝莓中分离出来，美国,并用作能够在蓝莓中诱发严重症状的参考菌株)。然而，植物中XfmIVIA5901的细菌种群显着低于XfmAlmaEm3。XfmESVL（从杏仁中分离出，阿利坎特,西班牙）和XfpXYL1961/18（从橄榄中分离出来，伊维萨岛,西班牙）引起的症状有限，而XfmXYL466/19(从野生橄榄中分离出来，马洛卡岛,西班牙）和XfmXF3348（从杏仁中分离出来，马洛卡岛,西班牙),和XfpDeDonno（与橄榄隔离，普利亚,意大利和破坏性橄榄快速下降综合症的代表）没有引起症状，也没有定植蓝莓。这项研究表明，如果满足有利于该地区寄主跳跃的条件，欧洲已经发现的某些菌株可能会感染蓝莓，例如，蓝莓与其他受感染的宿主的距离以及以这些作物为食的昆虫媒介的存在。需要对西班牙和其他欧洲国家的蓝莓中X.fastidiosa的存在进行调查，以预测可能的问题。
