PDF(Pubmed)
Abstract：
UNASSIGNED: Disruptions in tight junction (TJ) protein expression leading to duodenal epithelial barrier impairment may contribute to increased intestinal permeability, potentially playing a role in functional dyspepsia (FD) pathophysiology. Currently published studies evaluated the role of several TJ proteins in FD patients with inconsistent results. Therefore, we conducted this systematic review and metaanalysis to evaluate the duodenal mucosal expression of several TJ proteins in FD.
UNASSIGNED: We performed a systematic electronic search on PubMed, EMBASE, and Scopus using predefined keywords. Diagnosis of FD by Rome III or Rome IV criteria was considered acceptable. Full articles satisfying our inclusion and exclusion criteria were included. The principal summary outcome was the mean difference of several TJ proteins in FD patients and control subjects.
UNASSIGNED: A total of 8 and 5 studies were included in our qualitative and quantitative synthesis, respectively, with a total population of 666 participants, out of which 420 were FD patients. No significant differences were observed between FD patients and controls in the expression of claudin-1 (-0.102 [95% CI, -0.303, 0.099]), claudin-2 (0.161 [95% CI, -0.134, 0.456)], claudin-3 (0.278 [95% CI, -0.280, 0.837]), claudin-4 (0.045 [95% CI, -0.264, 0.354]), ZO-1 (-0.221 [95% CI, -0.683, 0.241]), ZO-2 (-0.070 [95% CI, -0.147, 0.007]), ZO-3 (-0.129 [95% CI, -0.376, 0.118]), β-catenin (-0.135 [95% CI, -0.484, 0.214]), E-cadherin (-0.083 [95% CI, -0.229, 0.063]), and occludin (-0.158 [95% CI, -0.409, 0.093]).
UNASSIGNED: The expressions of all evaluated proteins including claudin-1, claudin-2, claudin-3, claudin-4, ZO-1, ZO-2, ZO-3, β-catenin, E-cadherin, and occludin did not significantly differ between FD patients and controls. However, due to the limited number of included studies, results should be interpreted with caution.
UNASSIGNED: We performed a systematic electronic search on PubMed, EMBASE, and Scopus using predefined keywords. Diagnosis of FD by Rome III or Rome IV criteria was considered acceptable. Full articles satisfying our inclusion and exclusion criteria were included. The principal summary outcome was the mean difference of several TJ proteins in FD patients and control subjects.
UNASSIGNED: A total of 8 and 5 studies were included in our qualitative and quantitative synthesis, respectively, with a total population of 666 participants, out of which 420 were FD patients. No significant differences were observed between FD patients and controls in the expression of claudin-1 (-0.102 [95% CI, -0.303, 0.099]), claudin-2 (0.161 [95% CI, -0.134, 0.456)], claudin-3 (0.278 [95% CI, -0.280, 0.837]), claudin-4 (0.045 [95% CI, -0.264, 0.354]), ZO-1 (-0.221 [95% CI, -0.683, 0.241]), ZO-2 (-0.070 [95% CI, -0.147, 0.007]), ZO-3 (-0.129 [95% CI, -0.376, 0.118]), β-catenin (-0.135 [95% CI, -0.484, 0.214]), E-cadherin (-0.083 [95% CI, -0.229, 0.063]), and occludin (-0.158 [95% CI, -0.409, 0.093]).
UNASSIGNED: The expressions of all evaluated proteins including claudin-1, claudin-2, claudin-3, claudin-4, ZO-1, ZO-2, ZO-3, β-catenin, E-cadherin, and occludin did not significantly differ between FD patients and controls. However, due to the limited number of included studies, results should be interpreted with caution.
摘要：
■导致十二指肠上皮屏障损伤的紧密连接（TJ）蛋白表达的中断可能有助于增加肠通透性，可能在功能性消化不良（FD）病理生理学中起作用。目前发表的研究评估了几种TJ蛋白在FD患者中的作用，结果不一致。因此,我们进行了系统综述和荟萃分析，以评估FD中几种TJ蛋白的十二指肠粘膜表达。
■我们在PubMed上进行了系统的电子搜索，EMBASE,和Scopus使用预定义的关键字。根据罗马III或罗马IV标准诊断FD被认为是可接受的。纳入了符合我们纳入和排除标准的完整文章。主要总结结果是FD患者和对照组中几种TJ蛋白的平均差异。
■在我们的定性和定量综合中总共包括了8项和5项研究，分别,共有666名参与者，其中420例为FD患者。在FD患者和对照组之间，claudin-1的表达没有显着差异（-0.102[95％CI，-0.303，0.099]），claudin-2(0.161[95%CI，-0.134，0.456)]，claudin-3（0.278[95%CI，-0.280，0.837]），claudin-4(0.045[95%CI，-0.264，0.354])，ZO-1（-0.221[95%CI，-0.683，0.241]），ZO-2（-0.070[95%CI，-0.147，0.007]），ZO-3（-0.129[95%CI，-0.376，0.118]），β-连环蛋白（-0.135[95%CI，-0.484，0.214]），E-钙黏着蛋白（-0.083[95%CI，-0.229，0.063]），和闭塞蛋白（-0.158[95%CI，-0.409，0.093]）。
■所有评估蛋白质的表达，包括claudin-1，claudin-2，claudin-3，claudin-4，ZO-1，ZO-2，ZO-3，β-catenin，E-cadherin,和occludin在FD患者和对照组之间没有显着差异。然而,由于纳入研究的数量有限，结果应谨慎解释。
■我们在PubMed上进行了系统的电子搜索，EMBASE,和Scopus使用预定义的关键字。根据罗马III或罗马IV标准诊断FD被认为是可接受的。纳入了符合我们纳入和排除标准的完整文章。主要总结结果是FD患者和对照组中几种TJ蛋白的平均差异。
■在我们的定性和定量综合中总共包括了8项和5项研究，分别,共有666名参与者，其中420例为FD患者。在FD患者和对照组之间，claudin-1的表达没有显着差异（-0.102[95％CI，-0.303，0.099]），claudin-2(0.161[95%CI，-0.134，0.456)]，claudin-3（0.278[95%CI，-0.280，0.837]），claudin-4(0.045[95%CI，-0.264，0.354])，ZO-1（-0.221[95%CI，-0.683，0.241]），ZO-2（-0.070[95%CI，-0.147，0.007]），ZO-3（-0.129[95%CI，-0.376，0.118]），β-连环蛋白（-0.135[95%CI，-0.484，0.214]），E-钙黏着蛋白（-0.083[95%CI，-0.229，0.063]），和闭塞蛋白（-0.158[95%CI，-0.409，0.093]）。
■所有评估蛋白质的表达，包括claudin-1，claudin-2，claudin-3，claudin-4，ZO-1，ZO-2，ZO-3，β-catenin，E-cadherin,和occludin在FD患者和对照组之间没有显着差异。然而,由于纳入研究的数量有限，结果应谨慎解释。
