Abstract：
Bacillus tequilensis DZY 6715 was isolated from healthy leaves in Camellia oleifera, and the strain DZY 6715 significantly inhibited anthracnose disease resulting from Colletotrichum fructicola in C. oleifera, besides, its associated mechanism of disease resistance was explored. B. tequilensis DZY 6715 treatment controlled mycelial growth of C. fructicola in C. oleifera, and significantly decreased C. oleifera anthracnose incidence and disease index compared with the control group. B. tequilensis DZY 6715 has strong biofilm forming ability, and also secretes extracellular β-1, 3-glucanase and chitinase, which could cause cell membranes damage and increased cellular compound leakage. C.oleifera treated with DZY 6715 also effectively enhanced enzyme activities and stimulated the synthesis the substances related to phenylpropane metabolism and reactive oxygen metabolism. Moreover, transcript profiling analysis revealed more differentially expressed genes related to phenylpropanoid pathway metabolism and antioxidant system inducing by DZY 6715 compared with the control in C. oleifera. Thus, it can be concluded that B. tequilensis DZY 6715 is a suitable bio-control agent to control anthracnose disease in C. oleifera.
摘要：
从油茶的健康叶片中分离出了特奎尔芽孢杆菌DZY6715，菌株DZY6715显着抑制了油菜籽中的炭疽病，此外,探讨了其抗病的相关机制。B.telquilensisDZY6715处理控制了C.fucticola在C.oleifera中的菌丝生长，与对照组相比，油茶炭疽病发病率和病情指数明显降低。龙须菜DZY6715具有很强的生物膜形成能力，并分泌细胞外β-1，3-葡聚糖酶和几丁质酶，这可能导致细胞膜损伤和细胞化合物渗漏增加。用DZY6715处理的油菌还有效地增强了酶活性，并刺激了与苯丙烷代谢和活性氧代谢有关的物质的合成。此外，转录谱分析显示，与对照相比，DZY6715诱导的与苯丙素途径代谢和抗氧化系统相关的差异表达基因更多。因此，可以得出结论，龙舌兰芽孢杆菌DZY6715是一种合适的生物防治剂，可以控制油茶炭疽病。
