PDF(Pubmed)
Abstract：
BACKGROUND: Clostridium perfringens (C. perfringens) is an important zoonotic microorganism that can cause animal and human infections, however information about the prevalence status in wild birds of this pathogenic bacterium is currently limited.
RESULTS: In this study, 57 strains of C. perfringens were isolated from 328 fecal samples of wild birds. All the isolates were identified as type A and 70.18% of the isolates carried the cpb2 gene. Antimicrobial susceptibility testing showed that and 22.80% of the isolates were classified as multidrug-resistant strains. The MLST analysis of the 57 isolates from wild birds was categorized into 55 different sequence types (STs) and clustered into eight clonal complexes (CCs) with an average of 20.1 alleles and the Simpson Diversity index (Ds) of 0.9812, and revealed a high level of genetic diversity within the C. perfringens populations. Interestingly, the isolates from swan goose were clustered in the same CC while isolates from other bird species were more scattered suggesting that a potential difference in genetic diversity among the C. perfringens populations associated with different bird species.
CONCLUSIONS: C. perfringens exhibits a wide range of host adaptations, varying degrees of antimicrobial resistance, and a high degree of genetic diversity in wild birds. Understanding the prevalence, toxin type, antimicrobial resistance, and genetic diversity of C. perfringens in wildlife populations is essential for developing effective strategies for disease control and management.
RESULTS: In this study, 57 strains of C. perfringens were isolated from 328 fecal samples of wild birds. All the isolates were identified as type A and 70.18% of the isolates carried the cpb2 gene. Antimicrobial susceptibility testing showed that and 22.80% of the isolates were classified as multidrug-resistant strains. The MLST analysis of the 57 isolates from wild birds was categorized into 55 different sequence types (STs) and clustered into eight clonal complexes (CCs) with an average of 20.1 alleles and the Simpson Diversity index (Ds) of 0.9812, and revealed a high level of genetic diversity within the C. perfringens populations. Interestingly, the isolates from swan goose were clustered in the same CC while isolates from other bird species were more scattered suggesting that a potential difference in genetic diversity among the C. perfringens populations associated with different bird species.
CONCLUSIONS: C. perfringens exhibits a wide range of host adaptations, varying degrees of antimicrobial resistance, and a high degree of genetic diversity in wild birds. Understanding the prevalence, toxin type, antimicrobial resistance, and genetic diversity of C. perfringens in wildlife populations is essential for developing effective strategies for disease control and management.
摘要：
背景：产气荚膜梭菌（C.产气荚膜）是一种重要的人畜共患微生物，可引起动物和人类感染，然而，关于这种致病菌在野生鸟类中的流行状况的信息目前有限。
结果：在这项研究中，从328份野生鸟类粪便样品中分离出57株产气荚膜梭菌。所有分离株均鉴定为A型，70.18％的分离株携带cpb2基因。药敏试验显示,22.80%的菌株被分类为多重耐药菌株。对野生鸟类的57个分离株进行的MLST分析分为55种不同的序列类型（STs），并聚集为8个克隆复合物（CC），平均等位基因为20.1，辛普森多样性指数（Ds）为0.9812，并揭示了产气荚膜梭菌种群内的高水平遗传多样性。有趣的是,天鹅鹅的分离株聚集在同一CC中，而其他鸟类的分离株更分散，这表明与不同鸟类相关的产气荚膜梭菌种群之间的遗传多样性存在潜在差异。
结论：C.产气荚膜表现出广泛的宿主适应，不同程度的抗菌素耐药性，和野生鸟类的高度遗传多样性。了解患病率，毒素类型，抗菌素耐药性，野生动植物种群中产气荚膜梭菌的遗传多样性对于制定有效的疾病控制和管理策略至关重要。
结果：在这项研究中，从328份野生鸟类粪便样品中分离出57株产气荚膜梭菌。所有分离株均鉴定为A型，70.18％的分离株携带cpb2基因。药敏试验显示,22.80%的菌株被分类为多重耐药菌株。对野生鸟类的57个分离株进行的MLST分析分为55种不同的序列类型（STs），并聚集为8个克隆复合物（CC），平均等位基因为20.1，辛普森多样性指数（Ds）为0.9812，并揭示了产气荚膜梭菌种群内的高水平遗传多样性。有趣的是,天鹅鹅的分离株聚集在同一CC中，而其他鸟类的分离株更分散，这表明与不同鸟类相关的产气荚膜梭菌种群之间的遗传多样性存在潜在差异。
结论：C.产气荚膜表现出广泛的宿主适应，不同程度的抗菌素耐药性，和野生鸟类的高度遗传多样性。了解患病率，毒素类型，抗菌素耐药性，野生动植物种群中产气荚膜梭菌的遗传多样性对于制定有效的疾病控制和管理策略至关重要。
