Abstract：
To better understand the interaction between attenuated vaccines and host antiviral responses, we used bioinformatics and public transcriptomics data to analyze the immune response mechanisms of host cells after canine distemper virus (CDV) infection in Vero cells and screened for potential key effector factors. In this study, CDV-QN-1 infect with Vero cells at an MOI of 0.5, and total RNA was extracted from the cells 24 h later and reverse transcribed into cDNA. Transcriptome high-throughput sequencing perform using Illumina. The results showed that 438 differentially expressed genes were screened, of which 409 were significantly up-regulated and 29 were significantly down-regulated. Eight differentially expressed genes were randomly selected for RT-qPCR validation, and the change trend was consistent with the transcriptomics data. GO and KEGG analysis of differentially expressed genes revealed that most of the differentially expressed genes in CDV-QN-1 infection in the early stage were related to immune response and antiviral activity. The enriched signaling pathways mainly included the interaction between cytokines and cytokine receptors, the NF-kappa B signaling pathway, the Toll-like receptor signaling pathway, and the NOD-like receptor signaling pathway. This study provides a foundation for further exploring the pathogenesis of CDV and the innate immune response of host cells in the early stage of infection.
摘要：
为了更好地了解减毒疫苗与宿主抗病毒反应之间的相互作用，我们使用生物信息学和公共转录组学数据分析了犬瘟热病毒(CDV)感染Vero细胞后宿主细胞的免疫应答机制,并筛选了潜在的关键效应因子.在这项研究中,CDV-QN-1以0.5的MOI感染Vero细胞,24小时后从细胞中提取总RNA并逆转录为cDNA。转录组高通量测序使用Illumina进行。结果表明,筛选出438个差异表达基因,其中409个显著上调，29个显著下调。随机选择8个差异表达基因进行RT-qPCR验证,变化趋势与转录组学数据一致。对差异表达基因的GO和KEGG分析显示，早期CDV-QN-1感染中大多数差异表达基因与免疫反应和抗病毒活性有关。富集的信号通路主要包括细胞因子与细胞因子受体的相互作用,NF-κB信号通路，Toll样受体信号通路,和NOD样受体信号通路。本研究为进一步探讨CDV的发病机制和感染早期宿主细胞的固有免疫反应奠定了基础。
