Abstract：
Alternative strategies for controlling Staphylococcus aureus and other pathogens have been continuously investigated, with nisin, a bacteriocin widely used in the food industry as a biopreservative, gaining increasing attention. In addition to its antimicrobial properties, bacteriocins have significant effects on genome functionality even at inhibitory concentrations. This study investigated the impact of subinhibitory concentrations of nisin on S. aureus. Culturing in the presence of 0.625 μmol l-1 nisin, led to the increased relative expression of hla, saeR, and sarA, genes associated with virulence while expression of the sea gene, encoding staphylococcal enterotoxin A (SEA), decreased. In an in vivo experiment, Galleria mellonella larvae inoculated with S. aureus cultured in the presence of nisin exhibited 97% mortality at 72 h post-infection, compared to over 40% of larvae mortality in larvae infected with S. aureus. A comprehensive understanding of the effect of nisin on the transcriptional response of virulence genes and the impact of these changes on the virulence of S. aureus can contribute to assessing the application of this bacteriocin in food and medical contexts.
摘要：
不断研究控制金黄色葡萄球菌和其他病原体的替代策略，有了Nisin,一种细菌素，在食品工业中广泛用作生物防腐剂，获得越来越多的关注。除了其抗菌性能，即使在抑制浓度下，细菌素也对基因组功能具有显着影响。本研究调查了亚抑制浓度的乳酸链球菌素对金黄色葡萄球菌的影响。在0.625μmoll-1乳酸链球菌素存在下培养，导致hla的相对表达增加，saeR,还有Sara,在表达海基因时与毒力相关的基因，编码葡萄球菌肠毒素A（SEA），decreased.在体内实验中,感染后72小时，接种了在乳酸链球菌素存在下培养的金黄色葡萄球菌的海棠幼虫的死亡率为97％，相比之下，感染金黄色葡萄球菌的幼虫死亡率超过40％。全面了解乳链菌肽对毒力基因转录反应的影响以及这些变化对金黄色葡萄球菌毒力的影响，有助于评估这种细菌素在食品和医疗环境中的应用。
