Abstract：
Vibrio parahaemolyticus (V. parahaemolyticus) is a major foodborne pathogen, which can cause serious foodborne illnesses like diarrhoea. Rapid on-site detection of foodborne pathogens is an ideal way to respond to foodborne illnesses. Herein, we provide an electrochemical sensor for rapid on-site detection. This sensor utilized a pH-sensitive metal-oxide material for the concurrent isothermal amplification and label-free detection of nucleic acids. Based on a pH-sensitive hydrated iridium oxide oxyhydroxide film (HIROF), the electrode transforms the hydrogen ion compound generated during nucleic acid amplification into potential, so as to achieve a real-time detection. The results can be transmitted to a smartphone via Bluetooth. Moreover, HIROF was applied in nucleic acid device detection, with a super-Nernst sensitivity of 77.6 mV/pH in the pH range of 6.0-8.5, and the sensitivity showed the best results so far. Detection of V. parahaemolyticus by this novel method showed a detection limit of 1.0 × 103 CFU/mL, while the time consumption was only 30 min, outperforming real-time fluorescence loop-mediated isothermal amplification (LAMP). Therefore, the characteristics of compact, portable, and fast make the sensor more widely used in on-site detection.
摘要：
副溶血性弧菌(V.副溶血病)是一种主要的食源性病原体，会导致严重的食源性疾病，如腹泻。食源性病原体的快速现场检测是应对食源性疾病的理想方法。在这里，我们提供了一种用于快速现场检测的电化学传感器。该传感器利用pH敏感的金属氧化物材料进行核酸的同时等温扩增和无标记检测。基于对pH敏感的水合氧化铱羟基氧化物薄膜（HIROF），电极将核酸扩增过程中产生的氢离子化合物转化为电势，从而实现了实时检测。结果可以通过蓝牙传输到智能手机。此外，HIROF在核酸装置检测中的应用,在6.0-8.5的pH范围内，超能斯特灵敏度为77.6mV/pH，灵敏度显示出迄今为止最好的结果。通过这种新方法检测副溶血性弧菌的检出限为1.0×103CFU/mL，而消耗的时间只有30分钟，优于实时荧光环介导等温扩增（LAMP）。因此,紧凑的特点，便携式，使传感器在现场检测中的应用更加广泛。
