METHODS: We evaluated three commercial ELISA kits (DRG Instruments, IVD Research, and Bordier Affinity Products) to detect IgG antibodies against Strongyloides stercoralis assays utilizing serum samples from travelers with microscopically confirmed strongyloidiasis (n = 50) and other imported helminthic infections (n = 159) as well as healthy controls (n = 50).
RESULTS: The DRG, IVD, and Bordier assays showed sensitivities of 58.0%, 64.0%, and 56.0%, respectively. Specificity values were 96.0%, 96.0%, and 92.0% in healthy controls, and 67.3%, 62.9%, and 76.7% in cases with other helminth infections, respectively. Cross-reactions were mostly observed in cases with other nematodes (37.5%, 42.5%, and 20.0%, respectively), but also in trematode (33.3%, 38.1%, and 19.0%, respectively) and in cestode infections (25.0%, 30.0%, and 32.5%, respectively).
CONCLUSIONS: The study demonstrates the diagnostic limitations of serological assays to detect or exclude cases of strongyloidiasis in returning travelers, who frequently present with recent or acute infections.
方法:我们评估了三种商业ELISA试剂盒(DRGInstruments,IVD研究,和BordierAffinityProducts),以利用显微镜证实的圆线虫病(n=50)和其他输入性蠕虫感染(n=159)以及健康对照(n=50)的旅行者的血清样本来检测抗骨圆线虫的IgG抗体。
结果:DRG,IVD,Bordier检测显示灵敏度为58.0%,64.0%,和56.0%,分别。特异性值为96.0%,96.0%,健康对照组为92.0%,和67.3%,62.9%,其他蠕虫感染病例占76.7%,分别。在与其他线虫的病例中主要观察到交叉反应(37.5%,42.5%,和20.0%,分别),而且在吸虫中(33.3%,38.1%,和19.0%,分别)和在c虫感染中(25.0%,30.0%,32.5%,分别)。
结论:该研究表明,在返回的旅行者中检测或排除线圆线虫病的血清学检测的诊断局限性,经常出现近期或急性感染的人。