Abstract：
Young shoots of cereals are widely regarded as superfoods with health benefits attributed to their potential antioxidant activity and antioxidant-related effects (e.g. anticancer). The current study aimed to examine the chemical characteristics of Hordeum vulgare methanolic and aqueous extracts and assess their antioxidant activity using the DDPH and ORAC. Furthermore, the inhibitory effect of xanthine oxidase was screened. TLC bioautography was employed to determine the polarity of the compounds present in the extracts that exhibited the most potent free radical scavenging activity. Total flavonoid content of the methanolic and aqueous extracts was 0.14 mg QE/g and 0.012 mg QE/g, respectively. The antioxidant activity of the methanolic extract was found to be more potent, with a value of 0.97 ± 0.13 mmol TE/g than the aqueous extract which had no activity. This study presents novel findings on the xanthine inhibitory activity of H. vulgare. The methanolic extract demonstrated moderate inhibition of xanthine oxidase with a value of 23.24%. The results of our study were compared with the phytochemical and pharmacological analysis of Triticum aestivum, and further comparison was made with the data reported in the literature. Inconsistencies were observed in the chemical and pharmacological properties of H. vulgare, which could be a result of using herbal material harvested in different vegetative phases and various methods used for extraction. The findings of our study indicate that the timing of the harvest and extraction method may play crucial role in attaining the optimal phytochemical composition of H. vulgare, hence enhancing its pharmacological activity.
摘要：
谷物的嫩芽被广泛认为是具有健康益处的超级食品,归因于其潜在的抗氧化活性和抗氧化相关作用(例如抗癌)。当前的研究旨在检查大麦甲醇和水提取物的化学特性，并使用DDPH和ORAC评估其抗氧化活性。此外，筛选黄嘌呤氧化酶的抑制作用。使用TLC生物自谱分析来确定提取物中存在的化合物的极性，这些提取物表现出最有效的自由基清除活性。甲醇和水提取物的总黄酮含量为0.14mgQE/g和0.012mgQE/g，分别。发现甲醇提取物的抗氧化活性更有效，比没有活性的水提取物的值为0.97±0.13mmolTE/g。这项研究提出了关于H.vulgare黄嘌呤抑制活性的新发现。甲醇提取物显示了对黄嘌呤氧化酶的中等抑制作用，值为23.24％。我们的研究结果与小麦的植物化学和药理分析进行了比较，并与文献报道的数据进行了进一步比较。观察到H.vulgare的化学和药理特性不一致，这可能是使用在不同营养阶段收获的草药材料和用于提取的各种方法的结果。我们的研究结果表明，收获和提取方法的时机可能在获得H.vulgare的最佳植物化学成分中起着至关重要的作用。从而增强其药理活性。
