Abstract：
Introduction. Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as plasma medicine for treating various diseases, including infections caused by Candida albicans, an opportunistic pathogen that can overgrow in individuals with weakened immune system.Gap Statement. To date, there has been less molecular study on cold plasma-treated C. albicans.Research Aim. The study aims to fill the gap in understanding the molecular response of C. albicans to cold plasma treatment.Methodology. This project involved testing a cold plasma generator to determine its antimicrobial effectiveness on C. albicans\' planktonic cells. Additionally, the cells\' transcriptomics responses were investigated using RNA sequencing at various treatment durations (1, 3 and 5 min).Results. The results show that our cold plasma effectively eliminates C. albicans. Cold plasma treatment resulted in substantial downregulation of important pathways, such as \'nucleotide metabolism\', \'DNA replication and repair\', \'cell growth\', \'carbohydrate metabolism\' and \'amino acid metabolism\'. This was an indication of cell cycle arrest of C. albicans to preserve energy consumption under unfavourable conditions. Nevertheless, C. albicans adapted its GSH antioxidant system to cope with the oxidative stress induced by reactive oxygen species, reactive nitrogen species and other free radicals. The treatment likely led to a decrease in cell pathogenicity as many virulence factors were downregulated.Conclusion. The study demonstrated the major affected pathways in cold plasma-treated C. albicans, providing valuable insights into the molecular response of C. albicans to cold plasma treatment. The findings contribute to the understanding of the antimicrobial efficiency of cold plasma and its potential applications in the field of microbiology.
摘要：
Introduction.冷等离子体经常用于消除微生物污染物的目的。在最优条件下,它可以作为治疗各种疾病的血浆药物，包括白色念珠菌引起的感染,一种机会性病原体，可以在免疫系统较弱的个体中过度生长。差距声明。迄今为止,对冷等离子体处理的白色念珠菌的分子研究较少。研究目标。该研究旨在填补在理解白色念珠菌对冷等离子体处理的分子响应方面的空白。方法论。该项目涉及测试冷等离子体发生器，以确定其对白色念珠菌浮游细胞的抗菌效果。此外,在不同的治疗持续时间(1,3和5分钟)使用RNA测序研究细胞转录组学反应。结果。结果表明,我们的冷等离子体有效地消除了白色念珠菌。冷等离子体处理导致重要途径的大幅下调，如“核苷酸代谢”，\'DNA复制和修复\',\'细胞生长\',“碳水化合物代谢”和“氨基酸代谢”。这表明白色念珠菌的细胞周期停滞以保持不利条件下的能量消耗。然而，白色念珠菌适应其GSH抗氧化系统以应对活性氧引起的氧化应激，活性氮和其他自由基。该处理可能导致细胞致病性降低,因为许多毒力因子被下调。结论。该研究证明了冷等离子体处理的白色念珠菌的主要受影响途径，为白色念珠菌对冷等离子体处理的分子响应提供有价值的见解。这些发现有助于理解冷等离子体的抗菌效率及其在微生物学领域的潜在应用。
