Abstract：
Lingzhi or reishi mushroom, Ganoderma lucidum, is a medicinal mushroom quite widely developed as herbal medicine because it has acted as an anticancer, antitumor, antioxidant, and anti-inflammatory. The active mycochemical compounds of G. lucidum mushrooms, such as flavonoids and polysaccharides, can suppress the release of pro-inflammatory cytokines and prevent lipid peroxidation due to oxidative stress. Rheumatoid arthritis (RA) is an autoimmune disease where the exact cause is unknown, and RA prevalence continues to increase yearly. In patients with RA, joint damage and inflammation occur. This study aims to evaluate the effectiveness of G. lucidum nanogels as anti-arthritis, anti-inflammatory, and antioxidative. The research method was a true experiment using a control group and treatment group that randomly assigned, using 24 male Wistar rats (Rattus norvegicus) induced with complete Freund\'s adjuvant (CFA) 0.1 mL. The rats were divided into six groups; healthy control/HCt (did not receive the treatment), negative control/NCt (induced by CFA), and positive control/PCt (given 0.012 diclofenac sodium). TG1 (given 250 mg G. lucidum nanogels), TG2 (given 500 mg G. lucidum nanogels), TG3 (given 750 mg G. lucidum nanogels). IgG, eNOS, IL-1β, COX-2, NOS, TNF-α, and IL-6 parameters were measured using ELISA, and the data obtained were analyzed by one-way ANOVA using SPSS (P < 0.05). The results showed that administering G. lucidum nanogels significantly reduced IgG, NOS, TNF-α, COX-2, IL-1β, and IL-6 and increased eNOS levels. The anti-inflammatory and antioxidative activities in suppressing pro-inflammatory cytokines and increasing eNOS levels prove that the nanogel extract G. lucidum have the potential to be developed as anti-arthritis natural therapeutic.
摘要：
灵芝或灵芝，灵芝,是一种作为草药被广泛开发的药用蘑菇，因为它具有抗癌作用，抗肿瘤，抗氧化剂，和抗炎。灵芝蘑菇的活性真菌化合物，如类黄酮和多糖，可以抑制促炎细胞因子的释放并防止由于氧化应激引起的脂质过氧化。类风湿性关节炎（RA）是一种自身免疫性疾病，确切原因未知，RA患病率逐年增加。在RA患者中，关节损伤和炎症发生。本研究旨在评估灵芝纳米凝胶作为抗关节炎的有效性，抗炎,和抗氧化。研究方法是一个真实的实验，使用对照组和治疗组，随机分配，使用完全弗氏佐剂（CFA）0.1mL诱导的24只雄性Wistar大鼠（Rattusnorvegicus）。将大鼠分为六组；健康对照/HCt（未接受治疗），阴性对照/NCt（CFA诱导），和阳性对照/PCt（给予0.012双氯芬酸钠）。TG1（给予250毫克灵芝纳米凝胶），TG2(给予500毫克灵芝纳米凝胶)，TG3(给予750mg灵芝纳米凝胶)。IgG,eNOS,IL-1β,COX-2,NOS,TNF-α,使用ELISA测量IL-6参数，数据采用SPSS单因素方差分析(P<0.05)。结果表明，施用灵芝纳米凝胶显著降低IgG，NOS,TNF-α,COX-2，IL-1β，和IL-6并增加eNOS水平。在抑制促炎细胞因子和增加eNOS水平方面的抗炎和抗氧化活性证明了纳米凝胶提取物G.lucidum具有开发作为抗关节炎天然治疗剂的潜力。
