Abstract：
Drug-resistant bacterial infections and their lipopolysaccharide-related inflammatory complications continue to pose significant challenges in traditional treatments. Inspired by the rapid initiation of resident macrophages to form aggregates for efficient antibacterial action, this study proposes a multifunctional and enhanced antibacterial strategy through the construction of novel biomimetic cell membrane polypeptide nanonets (R-DPB-TA-Ce). The design involves the fusion of end-terminal lipidated polypeptides containing side-chain cationic boronic acid groups (DNPLBA) with cell membrane intercalation engineering (R-DPB), followed by coordination with the tannic acid-cerium complex (TA-Ce) to assemble into a biomimetic nanonet through boronic acid-polyphenol-metal ion interactions. In addition to the ability of RAW 264.7 macrophages cell membrane components\' (R) ability to neutralize lipopolysaccharide (LPS), R-DPB-TA-Ce demonstrated enhanced capture of bacteria and its LPS, leveraging nanoconfinement-enhanced multiple interactions based on the boronic acid-polyphenol nanonets skeleton combined with polysaccharide. Utilizing these advantages, indocyanine green (ICG) is further employed as a model drug for delivery, showcasing the exceptional treatment effect of R-DPB-TA-Ce as a new biomimetic assembled drug delivery system in antibacterial, anti-inflammatory, and wound healing promotion. Thus, this strategy of mimicking macrophage aggregates is anticipated to be further applicable to various types of cell membrane engineering for enhanced antibacterial treatment.
摘要：
耐药细菌感染及其脂多糖相关的炎症并发症继续对传统治疗提出重大挑战。受常驻巨噬细胞快速启动形成有效抗菌作用的聚集体的启发，本研究通过构建新型仿生细胞膜多肽纳米网（R-DPB-TA-Ce），提出了一种多功能和增强的抗菌策略。该设计涉及包含侧链阳离子硼酸基团（DNPLBA）的末端脂化多肽与细胞膜嵌入工程（R-DPB）的融合，然后与单宁酸-铈络合物（TA-Ce）配位，通过硼酸-多酚-金属离子相互作用组装成仿生纳米网。除了RAW264.7巨噬细胞细胞膜成分中和脂多糖（LPS）的能力，R-DPB-TA-Ce增强了对细菌及其LPS的捕获，利用基于硼酸-多酚纳米网骨架与多糖结合的纳米约束增强的多重相互作用。利用这些优势，吲哚菁绿（ICG）进一步用作模型药物用于递送，展示了R-DPB-TA-Ce作为一种新型仿生组装给药系统在抗菌领域的卓越治疗效果,抗炎,和促进伤口愈合。因此，这种模拟巨噬细胞聚集体的策略预计将进一步适用于各种类型的细胞膜工程，以增强抗菌治疗。
