PDF(Pubmed)
Abstract：
UNASSIGNED: Peach allergy is common food allergen. Allergen components-specific antibodies of different isotypes in peach-allergy patients are poorly studied. Factors other than Pru p 3-sIgE levels may be related to severe symptoms.
UNASSIGNED: To evaluated peach component-specific-IgE, IgG1, and IgG4 characteristics in individuals with and without peach allergy, and Pru p 3-sIgE affinity in patients with different clinical symptoms.
UNASSIGNED: Fifteen healthy controls and 32 peach-allergy patients were enrolled. sIgE, sIgG1, and sIgG4 to 5 Escherichia coli-expressed peach-allergen components were determined by enzyme-linked immunosorbent assays. Pru p 3-sIgE affinity was measured in Pru p 3-sIgE-positive patients, using immunoadsorbance.
UNASSIGNED: Patients were divided into oral allergy syndrome (OAS) and peach-induced anaphylaxis (PIA) groups. Serum Pru p 1-, Pru p 2-, Pru p 3-, Pru p 4-, and Pru p 7-sIgG1s were detected. Pru p 1- and Pru p 2-sIgG1 levels were higher in healthy controls, but Pru p 3-sIgG1 levels were significantly higher in peach-allergy patients. Pru p 1-, Pru p 3-, and Pru p 4-sIgG4-positivity was significantly greater among patients than among controls. Pru p 3 was the predominant allergen in peach-allergy patients. Allergen-sIgG1 and sIgG4 were similar between OAS and PIA patients. Pru p 3-sIgE levels were significantly higher in PIA patients, but Pru p 3-sIgE-positivity was similar in both groups. In Pru p 3-sIgE-positive patients, Pru p 3-sIgE affinity was significantly higher in PIA than OAS patients.
UNASSIGNED: Allergen-sIgG1 was associated with allergen exposure. Both Pru p 3-sIgE levels and affinity are key factors in severe peach-allergy patients.
UNASSIGNED: To evaluated peach component-specific-IgE, IgG1, and IgG4 characteristics in individuals with and without peach allergy, and Pru p 3-sIgE affinity in patients with different clinical symptoms.
UNASSIGNED: Fifteen healthy controls and 32 peach-allergy patients were enrolled. sIgE, sIgG1, and sIgG4 to 5 Escherichia coli-expressed peach-allergen components were determined by enzyme-linked immunosorbent assays. Pru p 3-sIgE affinity was measured in Pru p 3-sIgE-positive patients, using immunoadsorbance.
UNASSIGNED: Patients were divided into oral allergy syndrome (OAS) and peach-induced anaphylaxis (PIA) groups. Serum Pru p 1-, Pru p 2-, Pru p 3-, Pru p 4-, and Pru p 7-sIgG1s were detected. Pru p 1- and Pru p 2-sIgG1 levels were higher in healthy controls, but Pru p 3-sIgG1 levels were significantly higher in peach-allergy patients. Pru p 1-, Pru p 3-, and Pru p 4-sIgG4-positivity was significantly greater among patients than among controls. Pru p 3 was the predominant allergen in peach-allergy patients. Allergen-sIgG1 and sIgG4 were similar between OAS and PIA patients. Pru p 3-sIgE levels were significantly higher in PIA patients, but Pru p 3-sIgE-positivity was similar in both groups. In Pru p 3-sIgE-positive patients, Pru p 3-sIgE affinity was significantly higher in PIA than OAS patients.
UNASSIGNED: Allergen-sIgG1 was associated with allergen exposure. Both Pru p 3-sIgE levels and affinity are key factors in severe peach-allergy patients.
摘要：
■桃子过敏是常见的食物过敏原。桃子过敏患者中不同同种型的过敏原成分特异性抗体研究甚少。除Prup3-sIgE水平外的其他因素可能与严重症状有关。
■要评估桃子成分特异性IgE，有和没有桃子过敏的个体的IgG1和IgG4特征，和Prup3-sIgE在不同临床症状患者中的亲和力。
■招募了15名健康对照和32名桃子过敏患者。sIgE,sIgG1和sIgG4对5种大肠杆菌表达的桃子变应原成分进行了酶联免疫吸附测定。在Prup3-sIgE阳性患者中测量Prup3-sIgE亲和力，使用免疫吸附。
■患者分为口腔过敏综合征（OAS）和桃子引起的过敏反应（PIA）组。血清Prup1-，Prup2-，Prup3-，Prup4-,并检测到Prup7-sIgG1。在健康对照组中，Prup1-和Prup2-sIgG1水平较高，但是桃子过敏患者的Prup3-sIgG1水平明显更高。普鲁p1-，Prup3-，患者中Prup4-sIgG4阳性显著高于对照组.Prup3是桃子过敏患者的主要过敏原。OAS和PIA患者的变应原sIgG1和sIgG4相似。PIA患者的Prup3-sIgE水平明显高于PIA患者，但两组Prup3-sIgE阳性相似.在Prup3-sIgE阳性患者中，PIA患者的Prup3-sIgE亲和力明显高于OAS患者。
■变应原-sIgG1与变应原暴露有关。Prup3-sIgE水平和亲和力都是严重桃子过敏患者的关键因素。
■要评估桃子成分特异性IgE，有和没有桃子过敏的个体的IgG1和IgG4特征，和Prup3-sIgE在不同临床症状患者中的亲和力。
■招募了15名健康对照和32名桃子过敏患者。sIgE,sIgG1和sIgG4对5种大肠杆菌表达的桃子变应原成分进行了酶联免疫吸附测定。在Prup3-sIgE阳性患者中测量Prup3-sIgE亲和力，使用免疫吸附。
■患者分为口腔过敏综合征（OAS）和桃子引起的过敏反应（PIA）组。血清Prup1-，Prup2-，Prup3-，Prup4-,并检测到Prup7-sIgG1。在健康对照组中，Prup1-和Prup2-sIgG1水平较高，但是桃子过敏患者的Prup3-sIgG1水平明显更高。普鲁p1-，Prup3-，患者中Prup4-sIgG4阳性显著高于对照组.Prup3是桃子过敏患者的主要过敏原。OAS和PIA患者的变应原sIgG1和sIgG4相似。PIA患者的Prup3-sIgE水平明显高于PIA患者，但两组Prup3-sIgE阳性相似.在Prup3-sIgE阳性患者中，PIA患者的Prup3-sIgE亲和力明显高于OAS患者。
■变应原-sIgG1与变应原暴露有关。Prup3-sIgE水平和亲和力都是严重桃子过敏患者的关键因素。
