PDF(Pubmed)
Abstract：
We previously showed through clinical trials that one plant-derived lactic acid bacteria (LAB) can improve constipation. We preliminarily found that the plant-derived LAB Lactococcus lactis BM32-1 can grow in a mixture of sericin and fibroin, which are extracted from silk and have been reported to help promote health. Thus, in the present study, we evaluated the favorable effect of a sericin/fibroin mixture (S/F-M), which was extracted from silk prepared from cocoons reared in an aseptic rearing system using an artificial diet, fermented with the BM32-1 strain through a clinical trial. The trial was conducted at Hiroshima University from June to October 2022 as a double-blind, placebo-controlled, randomized parallel-group comparative study with 50 eligible subjects (aged 23-71) who had an average defecation frequency of less than 5 times per week. The subjects were instructed to drink 100 mL of fermented S/F-M or placebo every day. After the 12 weeks of the clinical trial period, the average defecation frequency increased significantly-1.4 times higher than that at baseline in the test group-as compared with the placebo group. Furthermore, the fecal microbiota was also compared before and after treatment, revealing that intake of the fermented S/F-M significantly multiplied the relative abundance of the genera Enterococcus and Clostridium, which have been reported to contribute to the amelioration of constipation by improving the gut microbiota and producing butyric acid, respectively. In conclusion, the S/F-M fermented using the BM32-1 strain improves defecation frequency through alteration of the gut microbiota.
摘要：
我们先前通过临床试验表明，一种植物来源的乳酸菌（LAB）可以改善便秘。我们初步发现，植物来源的LAB乳酸乳球菌BM32-1可以在丝胶和丝心蛋白的混合物中生长，它们是从丝绸中提取的，据报道有助于促进健康。因此，在本研究中，我们评估了丝胶/丝心蛋白混合物（S/F-M）的有利效果，从使用人工饮食在无菌饲养系统中饲养的茧中制备的蚕丝中提取，通过临床试验用BM32-1菌株发酵。该试验于2022年6月至10月在广岛大学进行，安慰剂对照,随机平行组比较研究,纳入50名符合条件的受试者(23-71岁),这些受试者每周平均排便次数少于5次.指示受试者每天饮用100mL发酵的S/F-M或安慰剂。经过12周的临床试验，与安慰剂组相比,试验组的平均排便频率显著增加,为基线时的1.4倍.此外，还比较了治疗前后的粪便微生物群，揭示了发酵的S/F-M的摄入量显着增加了肠球菌和梭菌属的相对丰度，据报道，通过改善肠道微生物群和产生丁酸来改善便秘，分别。总之,使用BM32-1菌株发酵的S/F-M通过改变肠道微生物区来改善排便频率。
