RESULTS: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations.
CONCLUSIONS: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.
结果:C57BL/6J小鼠维持含有猪油的饮食,MLCT,或MCT以7%或30%w/w持续10周(每组n=6)。测量粪便脂肪酸浓度以量化未吸收的脂肪含量。通过流式细胞术测量粪便中IgA包被的细菌与总细菌的比率(IgA包被比率)。与猪油喂养的老鼠相比,MLCT和MCT喂养的小鼠表现出较低的粪便浓度的棕榈酸,硬脂酸,和油酸和更高的IgA涂层比例在7%和30%的膳食脂肪,这些参数表现出显著的负相关关系。
结论:肠道中未吸收的脂肪含量可能导致高猪油饮食小鼠的细菌IgA涂层减弱。