Abstract：
Harmful algal blooms (HABs) of Alexandrium pacificum have affected the Marlborough Sounds in New Zealand since 2010, posing a threat to green-lipped mussel (GLM, Perna canaliculus) farming. Previous studies have shown A. pacificum has negative effects GLM embryos and larvae. To further investigate these toxic mechanisms, in vitro bioassays were conducted on GLM spermatozoa, hemocytes, and the diatom, Chaetoceros muelleri. The three cell types were exposed to several treatments of A. pacificum for 2 h and responses were measured using flow cytometry and pulse amplitude-modulated fluorometry. Significant spermatozoa mortality was recorded in treatments containing A. pacificum cells or fragments, while hemocyte and C. muelleri mortality was recorded in cell-free treatments of A. pacificum which contained paralytic shellfish toxins (PSTs). Variation in sensitivity between cell types as well as the sublethal effects observed, emphasise the diverse toxic mechanisms of A. pacificum on co-occurring species in the environment.
摘要：
自2010年以来，Alexandriumpacificum的有害藻华（HAB）已经影响了新西兰的MarlboroughSounds，对绿唇贻贝（GLM，Pernacanaliculus）农业。先前的研究表明，A.pacificum对GLM胚胎和幼虫有负面影响。为了进一步研究这些毒性机制，对GLM精子进行了体外生物测定，血细胞，还有硅藻,Chaetocerosmuelleri.将三种细胞类型暴露于几种处理的A.pacificum2小时，并使用流式细胞术和脉冲幅度调制荧光法测量反应。在含有A.pacificum细胞或片段的治疗中记录了显着的精子死亡率，而血细胞和C.muelleri死亡率记录在含麻痹性贝类毒素（PST）的A.pacificum的无细胞处理中。细胞类型之间的敏感性变化以及观察到的亚致死效应，强调A.pacificum对环境中共存物种的多种毒性机制。
