Abstract：
Castration promotes subcutaneous fat deposition that may be associated with metabolic adaptations in the liver. However, fatty acid composition, abundance, and metabolic characteristics of the liver after castration are not fully understood. Our results showed that surgical castration significantly reduced water and food intake, reduced liver weight, and induced liver inflammation in mice. Transcriptome analyses revealed that castration enhanced fatty acid metabolism, particularly that of arachidonic and linoleic acids metabolism. Gas chromatography-mass spectrometry analysis revealed that castration altered the composition and relative abundance of fatty acids in the liver. The relative abundances of arachidonic and linoleic acids were significantly decreased in 4-week-old castrated mice. Analysis of fatty acid synthesis- and metabolism-related genes revealed that castration enhanced the transcription of fatty acid synthesis- and oxidation-related genes. Analyzing the level of key enzymes in the β-oxidation and tricarboxylic acid cycle pathways of fatty acids in mitochondria, we found that castration enhanced the β-oxidation of fatty acids in mitochondria, and also enhanced the protein level of the rate-limiting enzyme in the tricarboxylic acid cycle pathway, isocitrate dehydrogenase 2. These results comprehensively clarify metabolic changes in liver fatty acids after castration in mice of different ages and provide a reference for understanding castration-induced fat deposition from the perspective of liver fatty acid metabolism in male mice.
摘要：
去势促进皮下脂肪沉积，这可能与肝脏中的代谢适应有关。然而,脂肪酸组成，丰度,以及去势后肝脏的代谢特征尚未完全了解。我们的结果表明，手术去势显着减少水和食物的摄入量，减轻肝脏重量，并诱导小鼠肝脏炎症。转录组分析显示，去势增强脂肪酸代谢，特别是花生四烯酸和亚油酸的代谢。气相色谱-质谱分析显示，去势改变了肝脏中脂肪酸的组成和相对丰度。在4周龄的cast割小鼠中，花生四烯酸和亚油酸的相对丰度显着降低。对脂肪酸合成和代谢相关基因的分析表明，去势增强了脂肪酸合成和氧化相关基因的转录。分析线粒体脂肪酸的β-氧化和三羧酸循环途径中关键酶的水平,我们发现去势增强了线粒体中脂肪酸的β-氧化，并提高了三羧酸循环途径中限速酶的蛋白质水平，异柠檬酸脱氢酶2。这些结果全面阐明了不同年龄小鼠去势后肝脏脂肪酸的代谢变化,为从雄性小鼠肝脏脂肪酸代谢的角度认识去势诱导的脂肪沉积提供了参考。
