%0 Journal Article
%T Castration reshapes the liver by altering fatty acid composition and metabolism in male mice.
%A Yao H
%A Li D
%A Cao X
%A Han X
%A He J
%A Cheng D
%A Shang J
%A Song T
%A Zeng X
%J Biochem Biophys Res Commun
%V 727
%N 0
%D 2024 Jun 27
%M 38963983
%F 3.322
%R 10.1016/j.bbrc.2024.150319
%X Castration promotes subcutaneous fat deposition that may be associated with metabolic adaptations in the liver. However, fatty acid composition, abundance, and metabolic characteristics of the liver after castration are not fully understood. Our results showed that surgical castration significantly reduced water and food intake, reduced liver weight, and induced liver inflammation in mice. Transcriptome analyses revealed that castration enhanced fatty acid metabolism, particularly that of arachidonic and linoleic acids metabolism. Gas chromatography-mass spectrometry analysis revealed that castration altered the composition and relative abundance of fatty acids in the liver. The relative abundances of arachidonic and linoleic acids were significantly decreased in 4-week-old castrated mice. Analysis of fatty acid synthesis- and metabolism-related genes revealed that castration enhanced the transcription of fatty acid synthesis- and oxidation-related genes. Analyzing the level of key enzymes in the β-oxidation and tricarboxylic acid cycle pathways of fatty acids in mitochondria, we found that castration enhanced the β-oxidation of fatty acids in mitochondria, and also enhanced the protein level of the rate-limiting enzyme in the tricarboxylic acid cycle pathway, isocitrate dehydrogenase 2. These results comprehensively clarify metabolic changes in liver fatty acids after castration in mice of different ages and provide a reference for understanding castration-induced fat deposition from the perspective of liver fatty acid metabolism in male mice.