Abstract:
As an indispensable part of insects, intestinal symbiotic bacteria play a vital role in the growth and development of insects and their adaptability. Rhoptroceros cyatheae, the main pest of the relict plant Alsophila spinulosa, poses a serious threat to the development of the A. spinulosa population. In the present study, 16S rDNA and internal transcribed spacer high-throughput sequencing techniques were used to analyze the structure of intestinal microbes and the diversity of the insect feeding on two different plants, as well as the similarities between the intestinal microorganisms of R. cyatheae. The dominant bacteria of leaf endophytes were also compared based on the sequencing data. The results showed that Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla of intestinal bacteria, and Ascomycota was the dominant phylum of intestinal fungi. Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Methylobacterium-Methylorubrum, and Enterococcus were the dominant genera in the intestine of R. cyatheae feeding on two plants, and the relative abundance was significantly different between the two groups. Candida was the common dominant genus of intestinal fungi in the two groups, and no significant difference was observed in its abundance between the two groups. This showed that compared with the intestinal fungi of R. cyatheae, the abundance of the intestinal bacteria was greatly affected by food. The common core microbiota between the microorganisms in A. spinulosa leaves and the insect gut indicated the presence of a microbial exchange between the two. The network correlation diagram showed that the gut microbes of R. cyatheae feeding on Gymnosphaera metteniana were more closely related to each other, which could help the host to better cope with the adverse external environment. This study provides a theoretical basis for the adaptation mechanism of R. cyatheae and a new direction for the effective prevention and control of R. cyatheae.
摘要:
作为昆虫不可或缺的一部分,肠道共生菌在昆虫的生长发育和适应性中起着至关重要的作用。石竹,遗留植物的主要害虫,对棘刺种群的发展构成严重威胁。在本研究中,16SrDNA和内部转录间隔区高通量测序技术用于分析肠道微生物的结构和昆虫在两种不同植物上取食的多样性,以及夏枯草的肠道微生物之间的相似性。还基于测序数据比较了叶片内生菌的优势细菌。结果表明,变形杆菌,Firmicutes,放线菌是肠道细菌的优势门,子囊菌是肠道真菌的优势门。异型根瘤菌-新根瘤菌-副根瘤菌-根瘤菌,甲基细菌-甲基细菌,肠球菌是以两种植物为食的蓝藻肠道中的优势属,两组的相对丰度差异显著。念珠菌是两组中常见的肠道真菌的优势属,两组之间的丰度没有显着差异。这表明,与夏枯草的肠道真菌相比,肠道细菌的丰度受食物的影响很大。刺五加叶中微生物与昆虫肠道之间的共同核心微生物群表明两者之间存在微生物交换。网络相关图显示,以Gynosphaerametteniana为食的蓝藻肠道微生物之间的关系更为密切,这可以帮助宿主更好地应对不利的外部环境。本研究为蓝藻的适应机制研究提供了理论依据,为蓝藻的有效防控提供了新的方向。