PDF(Pubmed)
Abstract：
BACKGROUND: The calmodulin (CaM) and calmodulin-like (CML) proteins play regulatory roles in plant growth and development, responses to biotic and abiotic stresses, and other biological processes. As a popular fruit and ornamental crop, it is important to explore the regulatory mechanism of flower and fruit development of passion fruit.
RESULTS: In this study, 32 PeCaM/PeCML genes were identified from passion fruit genome and were divided into 9 groups based on phylogenetic analysis. The structural analysis, including conserved motifs, gene structure and homologous modeling, illustrates that the PeCaM/PeCML in the same subgroup have relative conserved structural features. Collinearity analysis suggested that the expansion of the CaM/CML gene family likely took place mainly by segmental duplication, and the whole genome replication events were closely related with the rapid expansion of the gene group. PeCaM/PeCMLs were potentially required for different floral tissues development. Significantly, PeCML26 had extremely high expression levels during ovule and fruit development compared with other PeCML genes, suggesting that PeCML26 had potential functions involved in the development of passion fruit flowers and fruits. The co-presence of various cis-elements associated with growth and development, hormone responsiveness, and stress responsiveness in the promoter regions of these PeCaM/PeCMLs might contribute to their diverse regulatory roles. Furthermore, PeCaM/PeCMLs were also induced by various abiotic stresses. This work provides a comprehensive understanding of the CaM/CML gene family and valuable clues for future studies on the function and evolution of CaM/CML genes in passion fruit.
CONCLUSIONS: A total of 32 PeCaM/PeCML genes were divided into 9 groups. The PeCaM/PeCML genes showed differential expression patterns in floral tissues at different development stages. It is worth noting that PeCML26, which is highly homologous to AtCaM2, not only interacts with multiple BBR-BPC TFs, but also has high expression levels during ovule and fruit development, suggesting that PeCML26 had potential functions involved in the development of passion fruit flowers and fruits. This research lays the foundation for future investigations and validation of the potential function of PeCaM/PeCML genes in the growth and development of passion fruit.
RESULTS: In this study, 32 PeCaM/PeCML genes were identified from passion fruit genome and were divided into 9 groups based on phylogenetic analysis. The structural analysis, including conserved motifs, gene structure and homologous modeling, illustrates that the PeCaM/PeCML in the same subgroup have relative conserved structural features. Collinearity analysis suggested that the expansion of the CaM/CML gene family likely took place mainly by segmental duplication, and the whole genome replication events were closely related with the rapid expansion of the gene group. PeCaM/PeCMLs were potentially required for different floral tissues development. Significantly, PeCML26 had extremely high expression levels during ovule and fruit development compared with other PeCML genes, suggesting that PeCML26 had potential functions involved in the development of passion fruit flowers and fruits. The co-presence of various cis-elements associated with growth and development, hormone responsiveness, and stress responsiveness in the promoter regions of these PeCaM/PeCMLs might contribute to their diverse regulatory roles. Furthermore, PeCaM/PeCMLs were also induced by various abiotic stresses. This work provides a comprehensive understanding of the CaM/CML gene family and valuable clues for future studies on the function and evolution of CaM/CML genes in passion fruit.
CONCLUSIONS: A total of 32 PeCaM/PeCML genes were divided into 9 groups. The PeCaM/PeCML genes showed differential expression patterns in floral tissues at different development stages. It is worth noting that PeCML26, which is highly homologous to AtCaM2, not only interacts with multiple BBR-BPC TFs, but also has high expression levels during ovule and fruit development, suggesting that PeCML26 had potential functions involved in the development of passion fruit flowers and fruits. This research lays the foundation for future investigations and validation of the potential function of PeCaM/PeCML genes in the growth and development of passion fruit.
摘要：
背景：钙调蛋白（CaM）和类钙调蛋白（CML）在植物生长和发育中起调节作用，对生物和非生物胁迫的反应，和其他生物过程。作为一种受欢迎的水果和观赏作物，探索百香果花果发育的调控机制具有重要意义。
结果：在这项研究中，从百香果基因组中鉴定出32个PeCaM/PeCML基因，并根据系统发育分析将其分为9组。结构分析,包括保守的图案，基因结构和同源建模，说明同一亚组中的PeCaM/PeCML具有相对保守的结构特征。共线性分析表明，CaM/CML基因家族的扩展可能主要是通过节段复制进行的，全基因组复制事件与基因组的快速扩增密切相关。不同花组织发育可能需要PeCaM/PeCML。重要的是,与其他PeCML基因相比，PeCML26在胚珠和果实发育过程中具有极高的表达水平，表明PeCML26具有参与百香果花和果实发育的潜在功能。与生长和发育相关的各种顺式元素的共存，激素反应性，这些PeCaM/PeCML的启动子区域中的应激反应可能有助于其不同的调节作用。此外，PeCaM/PeCML也被各种非生物胁迫诱导。这项工作提供了对CaM/CML基因家族的全面了解，并为以后研究百香果中CaM/CML基因的功能和进化提供了有价值的线索。
结论：共32个PeCaM/PeCML基因分为9组。PeCaM/PeCML基因在不同发育阶段的花组织中显示出差异表达模式。值得注意的是，与AtCaM2高度同源的PeCML26不仅与多个BBR-BPCTFs相互作用，而且在胚珠和果实发育过程中也有较高的表达水平，表明PeCML26具有参与百香果花和果实发育的潜在功能。本研究为进一步研究和验证PeCaM/PeCML基因在百香果生长发育中的潜在功能奠定了基础。
结果：在这项研究中，从百香果基因组中鉴定出32个PeCaM/PeCML基因，并根据系统发育分析将其分为9组。结构分析,包括保守的图案，基因结构和同源建模，说明同一亚组中的PeCaM/PeCML具有相对保守的结构特征。共线性分析表明，CaM/CML基因家族的扩展可能主要是通过节段复制进行的，全基因组复制事件与基因组的快速扩增密切相关。不同花组织发育可能需要PeCaM/PeCML。重要的是,与其他PeCML基因相比，PeCML26在胚珠和果实发育过程中具有极高的表达水平，表明PeCML26具有参与百香果花和果实发育的潜在功能。与生长和发育相关的各种顺式元素的共存，激素反应性，这些PeCaM/PeCML的启动子区域中的应激反应可能有助于其不同的调节作用。此外，PeCaM/PeCML也被各种非生物胁迫诱导。这项工作提供了对CaM/CML基因家族的全面了解，并为以后研究百香果中CaM/CML基因的功能和进化提供了有价值的线索。
结论：共32个PeCaM/PeCML基因分为9组。PeCaM/PeCML基因在不同发育阶段的花组织中显示出差异表达模式。值得注意的是，与AtCaM2高度同源的PeCML26不仅与多个BBR-BPCTFs相互作用，而且在胚珠和果实发育过程中也有较高的表达水平，表明PeCML26具有参与百香果花和果实发育的潜在功能。本研究为进一步研究和验证PeCaM/PeCML基因在百香果生长发育中的潜在功能奠定了基础。
