PDF(Pubmed)
Abstract：
OBJECTIVE: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is extensively employed for the identification of filamentous fungi on MALDI Biotyper (Bruker Daltonics) and Vitek MS (biomerieux), but the performance of fungi identification on new EXS2600 (Zybio) is still unknow. Our study aims to evaluate the new EXS2600 system\'s (Zybio) ability to rapidly identify filamentous fungi and determine its effect on turnaround time (TAT) in our laboratory.
METHODS: We tested 117 filamentous fungi using two pretreatment methods: the formic acid sandwich (FA-sandwich) and a commercial mold extraction kit (MEK, Zybio). All isolates were confirmed via sequence analysis. Laboratory data were extracted from our laboratory information system over two 9-month periods: pre-EXS (April to December 2022) and post-EXS (April to December 2023), respectively.
RESULTS: The total correct identification (at the species, genus, or complex/group level) rate of fungi was high, FA-sandwich (95.73%, 112/117), followed by MEK (94.02%, 110/117). Excluding 6 isolates not in the database, species-level identification accuracy was 92.79% (103/111) for FA-sandwich and 91.89% (102/111) for MEK; genus-level accuracy was 97.29% (108/111) and 96.39% (107/111), respectively. Both methods attained a 100% correct identification rate for Aspergillus, Lichtheimia, Rhizopus Mucor and Talaromyces species, and were able to differentiate between Fusarium verticillioides and Fusarium proliferatum within the Fusarium fujikuroi species complex. Notably, high confidence was observed in the species-level identification of uncommon fungi such as Trichothecium roseum and Geotrichum candidum. The TAT for all positive cultures decreased from pre EXS2600 to post (108.379 VS 102.438, P < 0.05), and the TAT for tissue decreased most (451.538 VS 222.304, P < 0.001).
CONCLUSIONS: The FA-sandwich method is more efficient and accurate for identifying filamentous fungi with EXS2600 than the MEK. Our study firstly evaluated the performance of fungi identification on EXS2600 and showed it is suitable for clinical microbiology laboratories use.
METHODS: We tested 117 filamentous fungi using two pretreatment methods: the formic acid sandwich (FA-sandwich) and a commercial mold extraction kit (MEK, Zybio). All isolates were confirmed via sequence analysis. Laboratory data were extracted from our laboratory information system over two 9-month periods: pre-EXS (April to December 2022) and post-EXS (April to December 2023), respectively.
RESULTS: The total correct identification (at the species, genus, or complex/group level) rate of fungi was high, FA-sandwich (95.73%, 112/117), followed by MEK (94.02%, 110/117). Excluding 6 isolates not in the database, species-level identification accuracy was 92.79% (103/111) for FA-sandwich and 91.89% (102/111) for MEK; genus-level accuracy was 97.29% (108/111) and 96.39% (107/111), respectively. Both methods attained a 100% correct identification rate for Aspergillus, Lichtheimia, Rhizopus Mucor and Talaromyces species, and were able to differentiate between Fusarium verticillioides and Fusarium proliferatum within the Fusarium fujikuroi species complex. Notably, high confidence was observed in the species-level identification of uncommon fungi such as Trichothecium roseum and Geotrichum candidum. The TAT for all positive cultures decreased from pre EXS2600 to post (108.379 VS 102.438, P < 0.05), and the TAT for tissue decreased most (451.538 VS 222.304, P < 0.001).
CONCLUSIONS: The FA-sandwich method is more efficient and accurate for identifying filamentous fungi with EXS2600 than the MEK. Our study firstly evaluated the performance of fungi identification on EXS2600 and showed it is suitable for clinical microbiology laboratories use.
摘要：
目的：基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）广泛用于MALDIBiotyper（BrukerDaltonics）和VitekMS（biomerieux）上丝状真菌的鉴定，但是真菌在新型EXS2600（Zybio）上的鉴定性能仍然未知。我们的研究旨在评估新的EXS2600系统（Zybio）快速识别丝状真菌的能力，并确定其对我们实验室的周转时间（TAT）的影响。
方法：我们使用两种预处理方法测试了117种丝状真菌：甲酸三明治（FA三明治）和商业霉菌提取试剂盒（MEK，Zybio）。通过序列分析确认所有分离物。实验室数据从我们的实验室信息系统中提取了两个9个月的时间：EXS之前（2022年4月至12月）和EXS之后（2023年4月至12月）。分别。
结果：总体正确的鉴定（在物种，属,或复杂/群体水平)真菌率高，FA-三明治(95.73%，112/117)，其次是MEK(94.02%，110/117)。不包括不在数据库中的6个隔离，FA三明治的种级识别准确率为92.79％（103/111），MEK的种级识别准确率为91.89％（102/111）；属级准确率为97.29％（108/111）和96.39％（107/111），分别。两种方法对曲霉的正确识别率均达到100％，Lichtheimia,毛霉根霉和Talaromces种，并且能够在富士镰刀菌物种复合体中区分旋切镰刀菌和增殖镰刀菌。值得注意的是,在罕见真菌的物种水平鉴定中观察到了很高的置信度，例如玫瑰毛霉和念珠菌。所有阳性培养物的TAT从EXS2600前下降到后（108.379VS102.438，P<0.05），组织TAT下降最多（451.538VS222.304，P<0.001）。
结论：FA夹心法比MEK更有效，更准确地鉴定EXS2600丝状真菌。我们的研究首先评估了在EXS2600上进行真菌鉴定的性能，并表明它适用于临床微生物学实验室。
方法：我们使用两种预处理方法测试了117种丝状真菌：甲酸三明治（FA三明治）和商业霉菌提取试剂盒（MEK，Zybio）。通过序列分析确认所有分离物。实验室数据从我们的实验室信息系统中提取了两个9个月的时间：EXS之前（2022年4月至12月）和EXS之后（2023年4月至12月）。分别。
结果：总体正确的鉴定（在物种，属,或复杂/群体水平)真菌率高，FA-三明治(95.73%，112/117)，其次是MEK(94.02%，110/117)。不包括不在数据库中的6个隔离，FA三明治的种级识别准确率为92.79％（103/111），MEK的种级识别准确率为91.89％（102/111）；属级准确率为97.29％（108/111）和96.39％（107/111），分别。两种方法对曲霉的正确识别率均达到100％，Lichtheimia,毛霉根霉和Talaromces种，并且能够在富士镰刀菌物种复合体中区分旋切镰刀菌和增殖镰刀菌。值得注意的是,在罕见真菌的物种水平鉴定中观察到了很高的置信度，例如玫瑰毛霉和念珠菌。所有阳性培养物的TAT从EXS2600前下降到后（108.379VS102.438，P<0.05），组织TAT下降最多（451.538VS222.304，P<0.001）。
结论：FA夹心法比MEK更有效，更准确地鉴定EXS2600丝状真菌。我们的研究首先评估了在EXS2600上进行真菌鉴定的性能，并表明它适用于临床微生物学实验室。
