PDF(Pubmed)
Abstract：
BACKGROUND: Accurate regulation of gene expression is crucial for normal development and function of cells. The prognostic significance and potential carcinogenic mechanisms of the related gene JARID2 in OSCC are not yet clear, but existing research has indicated a significant association between the two.
METHODS: The relationship between the expression of the JARID2 gene in tumor samples of OSCC patients and clinical pathological factors was analyzed using immunohistochemistry experiments and RT-qPCR analysis. Based on the clinical pathological data of patients, bioinformatics analysis was conducted using public databases to determine the function of JARID2 in OSCC. Knockdown OSCC cell lines were constructed, and the impact of JARID2 on the biological behavior of OSCC cell lines was assessed through CCK-8, wound healing assay, and transwell analysis.
RESULTS: Immunohistochemistry experiments confirmed the correlation between JARID2 and the prognosis of OSCC patients, while RT-qPCR experiments demonstrated its expression levels in tissue and cells. CKK-8 experiments, wound healing assays, and Transwell experiments indicated that knocking down JARID2 had a negative impact on the proliferation, invasion, and migration of OSCC cells. Bioinformatics analysis results showed that the expression of JARID2 in OSCC is closely associated with patient gene co-expression, gene function enrichment, immune infiltration, and drug sensitivity.
CONCLUSIONS: Our study indicates that JARID2 is a novel prognostic biomarker and potential therapeutic target for OSCC.
METHODS: The relationship between the expression of the JARID2 gene in tumor samples of OSCC patients and clinical pathological factors was analyzed using immunohistochemistry experiments and RT-qPCR analysis. Based on the clinical pathological data of patients, bioinformatics analysis was conducted using public databases to determine the function of JARID2 in OSCC. Knockdown OSCC cell lines were constructed, and the impact of JARID2 on the biological behavior of OSCC cell lines was assessed through CCK-8, wound healing assay, and transwell analysis.
RESULTS: Immunohistochemistry experiments confirmed the correlation between JARID2 and the prognosis of OSCC patients, while RT-qPCR experiments demonstrated its expression levels in tissue and cells. CKK-8 experiments, wound healing assays, and Transwell experiments indicated that knocking down JARID2 had a negative impact on the proliferation, invasion, and migration of OSCC cells. Bioinformatics analysis results showed that the expression of JARID2 in OSCC is closely associated with patient gene co-expression, gene function enrichment, immune infiltration, and drug sensitivity.
CONCLUSIONS: Our study indicates that JARID2 is a novel prognostic biomarker and potential therapeutic target for OSCC.
摘要：
背景：基因表达的准确调节对于细胞的正常发育和功能至关重要。相关基因JARID2在OSCC中的预后意义和潜在致癌机制尚不清楚，但现有的研究表明两者之间有显著的关联。
方法：采用免疫组化实验和RT-qPCR分析OSCC患者肿瘤标本中JARID2基因的表达与临床病理因素的关系。根据患者的临床病理资料,使用公共数据库进行生物信息学分析,以确定JARID2在OSCC中的功能.敲低OSCC细胞系的构建，并通过CCK-8，伤口愈合试验评估JARID2对OSCC细胞系生物学行为的影响，和Transwell分析。
结果：免疫组化实验证实了JARID2与OSCC患者预后的相关性，而RT-qPCR实验证明其在组织和细胞中的表达水平。CKK-8实验，伤口愈合试验，和Transwell实验表明，击倒JARID2对增殖有负面影响，入侵,和OSCC细胞的迁移。生物信息学分析结果显示,JARID2在OSCC中的表达与患者基因共表达密切相关,基因功能富集，免疫浸润，和药物敏感性。
结论：我们的研究表明JARID2是一种新型的OSCC预后生物标志物和潜在的治疗靶点。
方法：采用免疫组化实验和RT-qPCR分析OSCC患者肿瘤标本中JARID2基因的表达与临床病理因素的关系。根据患者的临床病理资料,使用公共数据库进行生物信息学分析,以确定JARID2在OSCC中的功能.敲低OSCC细胞系的构建，并通过CCK-8，伤口愈合试验评估JARID2对OSCC细胞系生物学行为的影响，和Transwell分析。
结果：免疫组化实验证实了JARID2与OSCC患者预后的相关性，而RT-qPCR实验证明其在组织和细胞中的表达水平。CKK-8实验，伤口愈合试验，和Transwell实验表明，击倒JARID2对增殖有负面影响，入侵,和OSCC细胞的迁移。生物信息学分析结果显示,JARID2在OSCC中的表达与患者基因共表达密切相关,基因功能富集，免疫浸润，和药物敏感性。
结论：我们的研究表明JARID2是一种新型的OSCC预后生物标志物和潜在的治疗靶点。
