PDF(Pubmed)
Abstract：
Inflammation is a biodefense mechanism that provides protection against painful conditions such as inflammatory bowel disease, other gastrointestinal problems, and irritable bowel syndrome. Paraprobiotics have probiotic characteristics of intestinal modulation along with merits of safety and stability. In this study, heat-killed Lactiplantibacillus plantarum KU15122 (KU15122) was investigated for its anti-inflammatory properties. KU15122 was subjected to heat-killed treatment for enhancement of its safety, and its concentration was set at 8 log CFU/mL for conducting different experiments. Nitric oxide production was most remarkably reduced in the KU15122 group, whereas it was increased in the LPS-treated group. In RAW 264.7 cells, KU15122 inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. ELISA revealed that among the tested strains, KU15122 exhibited the most significant reduction in PGE2, IL-1β, and IL-6. Moreover, KU15122 inhibited various factors involved in the nuclear factor-kappa B, activator protein-1, and mitogen-activated protein kinase pathways. In addition, KU15122 reduced the generation of reactive oxygen species. The anti-inflammatory effect of KU15122 was likely attributable to the bacterial exopolysaccharides. Conclusively, KU15122 exhibits anti-inflammatory potential against inflammatory diseases.
摘要：
炎症是一种生物防御机制，可提供针对炎症性肠病等疼痛状况的保护，其他肠胃问题,肠易激综合征.副益生菌具有肠道调节的益生菌特性以及安全性和稳定性的优点。在这项研究中,研究了热灭活的植物乳杆菌KU15122（KU15122）的抗炎特性。KU15122进行了热镇静处理，以提高其安全性，并且其浓度设定为8logCFU/mL以进行不同的实验。在KU15122组中，一氧化氮的产生最显著地减少，而在LPS处理组增加。在RAW264.7单元格中，KU15122抑制诱导型一氧化氮合酶的表达，环氧合酶-2，白细胞介素（IL）-1β，IL-6和肿瘤坏死因子-α。ELISA显示，在测试的菌株中，KU15122在PGE2、IL-1β、IL-6此外，KU15122抑制参与核因子-κB的各种因素，激活蛋白-1和丝裂原激活的蛋白激酶途径。此外,KU15122减少了活性氧的产生。KU15122的抗炎作用可能归因于细菌胞外多糖。最后，KU15122表现出对抗炎性疾病的抗炎潜力。
