Abstract：
Reducing the risk of wound infection is an urgent issue health priority. Antibacterial polysaccharide-based hydrogels have attracted great attention for infectious wounds, attributed to their safe antimicrobial performance and natural non-toxicity and biodegradability advantages. In this study, the \"all-in-one\" self-adaptive and injectable cationic guar gum (CG)-based polysaccharide hydrogels (FA-TOB/CG) loaded with bioactive complexes were developed for infectious wound healing. The constructed antioxidant and antibacterial ferulic acid (FA)-tobramycin (TOB) bioactive complexes (FA-TOB) were used as the cross-linking agent and introduced into the CG matrix to construct the FA-TOB/CG hydrogel with a three-dimensional porous structure. The sterilization rates of FA-TOB/CG hydrogel against S. aureus and E. coli reached 98 % and 80 % respectively. In addition, the FA-TOB/CG also exhibits enhanced antioxidant performances (DPPH: > 40 %; ABTS: > 90 %; ·OH: > 50 %). More importantly, FA-TOB/CG hydrogel also showed the ability to sustain the release of FA and TOB. These superiorities of the FA-TOB/CG hydrogel enabled it to provide a moist wound environment and promote wound healing by eliminating bacteria, modulating the local inflammatory response, and accelerating collagen deposition and vascular regeneration. Thus, this study may enlarge a new sight for developing multifunctional dressings by incorporating bioactive complexes into polysaccharide hydrogels for infected wounds.
摘要：
降低伤口感染的风险是一个紧迫的健康优先事项。基于抗菌多糖的水凝胶在感染性伤口中引起了极大的关注,归因于其安全的抗菌性能和天然无毒和可生物降解的优势。在这项研究中,开发了“多合一”自适应和可注射的阳离子瓜尔胶（CG）基多糖水凝胶（FA-TOB/CG），负载了生物活性复合物，用于感染性伤口愈合。以构建的抗氧化剂和抗菌阿魏酸(FA)-妥布霉素(TOB)生物活性复合物(FA-TOB)为交联剂,引入到CG基质中,构建具有三维多孔结构的FA-TOB/CG水凝胶。FA-TOB/CG水凝胶对金黄色葡萄球菌和大肠杆菌的杀菌率分别达到98%和80%。此外,FA-TOB/CG还表现出增强的抗氧化性能(DPPH:>40%;ABTS:>90%;·OH:>50%)。更重要的是,FA-TOB/CG水凝胶也显示出维持FA和TOB释放的能力。FA-TOB/CG水凝胶的这些优势使其能够提供湿润的伤口环境，并通过消除细菌促进伤口愈合，调节局部炎症反应，加速胶原沉积和血管再生.因此，这项研究可能会扩大一个新的视野，为开发多功能敷料将生物活性复合物掺入多糖水凝胶感染的伤口。
