Abstract：
Amyloid A (AA) amyloidosis is induced by administering amyloid fibrils to animals under inflammatory conditions. Silk fibroin (SF), the main component of silk threads, forms amyloid-like fibrils and has been previously reported to induce AA amyloidosis in mice. In this study, SF was cultured in ethanol solution, and after confirming fibril formation through Thioflavin T assay, Congo red assay, and observation under electron microscopy, cultured SF ethanol solutions were administered to mice via various routes to investigate the induction of target organs and amyloidosis. As a result, cultured SF ethanol solutions were confirmed to reach the lungs and spleen, but no amyloid deposition was observed. While SF forms amyloid-like fibril structures through cultivation in ethanol solution, its amyloid-enhancing factor (AEF) activity is considered low in mice.
摘要：
淀粉样蛋白A(AA)淀粉样变性是通过在炎症条件下向动物施用淀粉样原纤维来诱导的。丝素蛋白(SF),丝线的主要成分，形成淀粉样蛋白样纤维，并且先前已报道在小鼠中诱导AA淀粉样变性。在这项研究中,SF在乙醇溶液中培养，在通过硫磺素T测定确认原纤维形成后，刚果红测定，在电子显微镜下观察，通过各种途径向小鼠施用培养的SF乙醇溶液以研究靶器官和淀粉样变性的诱导。因此,培养的SF乙醇溶液被证实到达肺和脾，但没有观察到淀粉样蛋白沉积。虽然SF通过在乙醇溶液中培养形成淀粉样蛋白样纤维结构，其淀粉样蛋白增强因子（AEF）活性在小鼠中被认为较低。
