%0 Journal Article
%T Studies on the potential risk of amyloidosis from exposure to cultured fibril from silk fibroin.
%A Osawa S
%A Iwaide S
%A Kobayashi K
%A Oba R
%A Murakami T
%J Biomed Phys Eng Express
%V 0
%N 0
%D 2024 Jul 3
%M 38959872
暂无%R 10.1088/2057-1976/ad5e86
%X Amyloid A (AA) amyloidosis is induced by administering amyloid fibrils to animals under inflammatory conditions. Silk fibroin (SF), the main component of silk threads, forms amyloid-like fibrils and has been previously reported to induce AA amyloidosis in mice. In this study, SF was cultured in ethanol solution, and after confirming fibril formation through Thioflavin T assay, Congo red assay, and observation under electron microscopy, cultured SF ethanol solutions were administered to mice via various routes to investigate the induction of target organs and amyloidosis. As a result, cultured SF ethanol solutions were confirmed to reach the lungs and spleen, but no amyloid deposition was observed. While SF forms amyloid-like fibril structures through cultivation in ethanol solution, its amyloid-enhancing factor (AEF) activity is considered low in mice.