Abstract：
Due to the limitations of the current skin wound treatments, it is highly valuable to have a wound healing formulation that mimics the extracellular matrix (ECM) and mechanical properties of natural skin tissue. Here, a novel biomimetic hydrogel formulation has been developed based on a mixture of Agarose-Collagen Type I (AC) combined with skin ECM-related components: Dermatan sulfate (DS), Hyaluronic acid (HA), and Elastin (EL) for its application in skin tissue engineering (TE). Different formulations were designed by combining AC hydrogels with DS, HA, and EL. Cell viability, hemocompatibility, physicochemical, mechanical, and wound healing properties were investigated. Finally, a bilayered hydrogel loaded with fibroblasts and mesenchymal stromal cells was developed using the Ag-Col I-DS-HA-EL (ACDHE) formulation. The ACDHE hydrogel displayed the best in vitro results and acceptable physicochemical properties. Also, it behaved mechanically close to human native skin and exhibited good cytocompatibility. Environmental scanning electron microscopy (ESEM) analysis revealed a porous microstructure that allows the maintenance of cell growth and ECM-like structure production. These findings demonstrate the potential of the ACDHE hydrogel formulation for applications such as an injectable hydrogel or a bioink to create cell-laden structures for skin TE.
摘要：
由于目前皮肤创伤治疗方法的局限性,具有模拟天然皮肤组织的细胞外基质(ECM)和机械性能的伤口愈合制剂是非常有价值的。这里,一种新的仿生水凝胶制剂已经开发的基础上，琼脂糖-胶原蛋白I型（AC）与皮肤ECM相关的成分的混合物：硫酸皮肤素（DS），透明质酸(HA),和弹性蛋白（EL）在皮肤组织工程（TE）中的应用。通过将AC水凝胶与DS组合设计不同的配方，HA,和EL。细胞活力，血液相容性,物理化学,机械,和伤口愈合特性进行了研究。最后,使用Ag-ColI-DS-HA-EL(ACDHE)制剂开发负载有成纤维细胞和间充质基质细胞的双层水凝胶。ACDHE水凝胶显示出最佳的体外结果和可接受的物理化学性质。此外，它的机械行为接近人类天然皮肤，并表现出良好的细胞相容性。环境扫描电子显微镜（ESEM）分析显示出多孔的微观结构，可以维持细胞生长和ECM样结构的产生。这些发现证明了ACDHE水凝胶制剂用于诸如可注射水凝胶或生物墨水的应用以产生用于皮肤TE的载有细胞的结构的潜力。
