PDF(Pubmed)
Abstract：
Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and anti-inflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used: normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2-5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties.
摘要：
熊果苷在传统疗法中被用来治疗许多综合征，因为它具有抗微生物作用，抗氧化剂，和抗炎特性。本研究旨在评估熊果苷对偶氮甲烷（AOM）诱导的大鼠结肠异常隐窝灶（ACF）的化学预防作用。使用五组大鼠:正常对照组(大鼠皮下注射无菌磷酸盐缓冲盐水,每周一次,持续两周)和第2-5组,皮下注射15mg/kgAOM,每周一次,持续两周。AOM对照组和5-氟尿嘧啶（5-FU）对照组每天使用饲管口服10％吐温，持续8周。治疗组每天饲喂30和60mg/kg熊果苷,持续2个月。来自AOM对照组的ACF除了多层细胞外还具有异常的细胞核并且不存在杯状细胞。阴性对照组在基底位置显示球形细胞和细胞核。组织学检查显示来自5-FU参照组的结肠组织的AFC细胞数量减少。与AOM对照相比，熊果苷喂养的动物显示出增殖细胞核抗原（PCNA）的下调和Bax蛋白的上调。与AOM对照组相比，饲喂熊果苷的大鼠结肠组织匀浆中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显着增加。总之,熊果苷对大肠癌有治疗作用，解释为其显著降低ACF的能力，下调PCNA蛋白，并上调Bax蛋白。此外,熊果苷显著增加SOD和CAT，丙二醛（MDA）水平降低，这可能是由于其抗增殖和抗氧化特性。
