Abstract：
OBJECTIVE: This study aims to assess the synergistic effect of utilizing a bioceramic sealer, NeoPutty, with photobiomodulation (PBM) on dental pulp stem cells (DPSCs) for odontogenesis.
METHODS: Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a post hoc t-test was employed to examine and compare the mean values of the results.
RESULTS: The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs.
CONCLUSIONS: The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs.
CONCLUSIONS: Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues. How to cite this article: Alshawkani HA, Mansy M, Al Ankily M, et al. Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. J Contemp Dent Pract 2024;25(4):313-319.
METHODS: Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a post hoc t-test was employed to examine and compare the mean values of the results.
RESULTS: The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs.
CONCLUSIONS: The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs.
CONCLUSIONS: Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues. How to cite this article: Alshawkani HA, Mansy M, Al Ankily M, et al. Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. J Contemp Dent Pract 2024;25(4):313-319.
摘要：
目的：本研究旨在评估利用生物陶瓷密封剂的协同作用，NeoPutty,在牙髓干细胞（DPSC）上进行光生物调节（PBM）以进行牙本质发育。
方法：从健康个体提取的10个前磨牙中收集牙髓干细胞。使用倒相显微镜检测细胞形状和流式细胞术检测干细胞特异性表面抗原来表征牙髓干细胞。检查了三个实验组：NP组，PBM组,和组合的NP和PBM组。进行3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)实验以评估DPSC的活力。使用茜素红染色分析牙源性分化潜能，牙源性基因DMP-1、DSPP、和碱性磷酸酶（ALP），和蛋白质印迹分析检测BMP-2和RUNX-2蛋白表达。采用方差分析（ANOVA），然后进行事后t检验，以检查并比较结果的平均值。
结果:研究显示,当NP和PBM一起使用时,细胞活力显著提高。在联合组中，牙源性基因表达以及BMP-2和RUNX-2的蛋白表达显着增加。NeoPutty和PBM的联合作用在增强DPSCs的牙源性分化能力方面显着。
结论：NeoPutty和PBM的协同作用对DPSCs的细胞相容性和牙源性分化潜能产生了最积极的影响。
结论：创建创新的再生治疗方法，以有效和持久地修复受损的牙齿组织。如何引用这篇文章:AlshawkaniHA,MansyM,艾尔·安利·M，etal.牙髓干细胞对生物陶瓷牙齿密封剂和光生物调节反应的再生潜力：体外研究。JContempDentPract2024；25（4）：313-319。
方法：从健康个体提取的10个前磨牙中收集牙髓干细胞。使用倒相显微镜检测细胞形状和流式细胞术检测干细胞特异性表面抗原来表征牙髓干细胞。检查了三个实验组：NP组，PBM组,和组合的NP和PBM组。进行3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)实验以评估DPSC的活力。使用茜素红染色分析牙源性分化潜能，牙源性基因DMP-1、DSPP、和碱性磷酸酶（ALP），和蛋白质印迹分析检测BMP-2和RUNX-2蛋白表达。采用方差分析（ANOVA），然后进行事后t检验，以检查并比较结果的平均值。
结果:研究显示,当NP和PBM一起使用时,细胞活力显著提高。在联合组中，牙源性基因表达以及BMP-2和RUNX-2的蛋白表达显着增加。NeoPutty和PBM的联合作用在增强DPSCs的牙源性分化能力方面显着。
结论：NeoPutty和PBM的协同作用对DPSCs的细胞相容性和牙源性分化潜能产生了最积极的影响。
结论：创建创新的再生治疗方法，以有效和持久地修复受损的牙齿组织。如何引用这篇文章:AlshawkaniHA,MansyM,艾尔·安利·M，etal.牙髓干细胞对生物陶瓷牙齿密封剂和光生物调节反应的再生潜力：体外研究。JContempDentPract2024；25（4）：313-319。
